静止培养的3T3成纤维细胞复制前期激素相互作用的时间顺序。
Temporal sequence of hormonal interactions during the prereplicative phase of quiescent cultured 3T3 fibroblasts.
作者信息
de Asua L J, O'Farrell M K, Clingan D, Rudland P S
出版信息
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3845-9. doi: 10.1073/pnas.74.9.3845.
Abstract
After addition of prostaglandin F2alpha (PGF2alpha) and insulin to quiescent cultured 3T3 fibroblasts a constant lag phase of 15 hr occurs before an increased rate of cellular exit from G1 is observed. The latter process follows first order kinetics, which can be quantified by a rate constant k. The temporal relationship of the interactions of PGF2 alpha, insulin, and hydrocortisone to produce alterations in the rate of exit from G1 was investigated. PGF2alpha establishes a constant lag phase and produces alterations in the rate constant k. These two effects can be partially separated by adding two concentrations of PGF2alpha at different times. Insulin fails to establish the lag phase but can stimulate the effect of PGF2alpha when added at any time after PGF2alpha. Hydrocortisone reduces the value of k when added between 0 and 3 hr after PGF2alpha. These results show that the lag phase can beseparated into temporal regions during which hormones can interact to produce changes in the rate of cellular exit from G1.
摘要
向静止培养的3T3成纤维细胞中添加前列腺素F2α（PGF2α）和胰岛素后，在观察到细胞从G1期退出的速率增加之前，会出现15小时的恒定延迟期。后一过程遵循一级动力学，可用速率常数k进行量化。研究了PGF2α、胰岛素和氢化可的松相互作用以改变从G1期退出速率的时间关系。PGF2α建立一个恒定的延迟期并改变速率常数k。通过在不同时间添加两种浓度的PGF2α，这两种效应可以部分分离。胰岛素未能建立延迟期，但在PGF2α之后的任何时间添加时，可刺激PGF2α的作用。在PGF2α之后0至3小时之间添加氢化可的松会降低k值。这些结果表明，延迟期可分为不同的时间段，在此期间激素可相互作用，从而改变细胞从G1期退出的速率。
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