Cordeiro Kátia Wolff, Felipe Josyelen Lousada, Malange Kauê Franco, do Prado Pâmela Rafaela, de Oliveira Figueiredo Patrícia, Garcez Fernanda Rodrigues, de Cássia Freitas Karine, Garcez Walmir Silva, Toffoli-Kadri Mônica Cristina
Faculdade de Medicina, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, Brazil.
Centro de Ciências Biológicas e da Saúde, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, Brazil.
J Ethnopharmacol. 2016 May 13;183:128-135. doi: 10.1016/j.jep.2016.02.051. Epub 2016 Mar 2.
Croton urucurana (Euphorbiaceae) is popularly used in Brazil to treat inflammatory processes, pain, and gastric ulcers.
To evaluate the anti-inflammatory and antinociceptive properties of the methanol extract from the bark of C. urucurana (MECu) in mice and identify its chemical constituents.
The extract was characterized by UHPLC-DAD-ESI-Q-TOF-MS/MS. Extract doses of 25, 100, and 400mg/kg were employed in the biological assays. Evaluation of anti-inflammatory activity was based on paw edema and leukocyte recruitment into the peritoneal cavity of mice, both induced by carrageenan. Abdominal writhing caused by acetic acid and duration of formalin-induced paw-licking were the models employed to evaluate antinociceptive activity.
Ten compounds were identified in the extract: (+)-gallocatechin (1), procyanidin B3 (2), (+)-catechin (3), (-)-epicatechin (4), tembetarine (5), magnoflorine (6), taspine (7), methyl-3-oxo-12-epi-barbascoate (8), methyl-12-epi-barbascoate (9), and hardwickiic acid (10). This is the first report of compounds 2, 4, 6, 7, and 10 in C. urucurana and compound 5 in the genus Croton. In addition to inhibiting paw edema and leukocyte recruitment (particularly of polymorphonuclear cells) into the peritoneal cavity of mice, MECu reduced the number of abdominal writhings induced by acetic acid and the duration of formalin-induced paw licking.
The methanol extract of C. urucurana bark exhibited anti-inflammatory and antinociceptive properties, corroborating its use in folk medicine. These effects may be related to the presence of diterpenes, alkaloids, and flavonoids.
巴豆属植物乌鲁库拉纳巴豆（大戟科）在巴西被广泛用于治疗炎症、疼痛和胃溃疡。
评估乌鲁库拉纳巴豆树皮甲醇提取物（MECu）对小鼠的抗炎和镇痛特性，并鉴定其化学成分。
采用超高效液相色谱-二极管阵列检测器-电喷雾电离-四极杆飞行时间串联质谱（UHPLC-DAD-ESI-Q-TOF-MS/MS）对提取物进行表征。在生物学试验中使用的提取物剂量为25、100和400mg/kg。基于角叉菜胶诱导的小鼠爪肿胀和白细胞募集到腹腔来评估抗炎活性。采用乙酸诱导的腹部扭体和福尔马林诱导的舔爪持续时间作为评估镇痛活性的模型。
提取物中鉴定出10种化合物：（+）-没食子儿茶素（1）、原花青素B3（2）、（+）-儿茶素（3）、（-）-表儿茶素（4）、坦贝他林（5）、木兰碱（6）、塔斯平碱（7）、甲基-3-氧代-12-表巴巴斯可酸酯（8）、甲基-12-表巴巴斯可酸酯（9）和哈德威克酸（10）。这是乌鲁库拉纳巴豆中化合物2、4、6、7和10以及巴豆属中化合物5的首次报道。除了抑制小鼠爪肿胀和白细胞募集（特别是多形核细胞）到腹腔外，MECu还减少了乙酸诱导的腹部扭体次数和福尔马林诱导的舔爪持续时间。
乌鲁库拉纳巴豆树皮甲醇提取物具有抗炎和镇痛特性，证实了其在民间医学中的应用。这些作用可能与二萜、生物碱和黄酮类化合物的存在有关。
