Kheradmandi Mahsa, Vasheghani-Farahani Ebrahim, Ghiaseddin Ali, Ganji Fariba
Biomedical Engineering Division, Chemical Engineering Faculty, Tarbiat Modares University, P.O. Box: 14115-143, Tehran, Iran.
J Biomed Mater Res A. 2016 Jul;104(7):1720-7. doi: 10.1002/jbm.a.35702. Epub 2016 Mar 20.
Skeletal muscle tissue shows a remarkable potential in regeneration of injured tissue. However, in some of chronic and volumetric muscle damages, the native tissue is incapable to repair and remodeling the trauma. In the same condition, stem-cell therapy increased regeneration in situations of deficient muscle repair, but the major problem seems to be the lack of ability to attachment and survive of injected cells on the exact location. In this study, chitosan/poly(vinyl alcohol) nanofibrous scaffold was studied to promote cell attachment and provide mechanical support during regeneration. Scaffold was characterized using scanning electron microscope, X-ray diffraction, and tensile test. Degradation and swelling behavior of scaffold were studied for 20 days. The cell-scaffold interaction was characterized by MTT assay for 10 days and in vivo biocompatibility of scaffold in a rabbit model was evaluated. Results showed that cells had a good viability, adhesion, growth, and spread on the scaffold, which make this mat a desirable engineered muscular graft. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1720-1727, 2016.
骨骼肌组织在损伤组织的再生方面显示出显著的潜力。然而，在一些慢性和大面积肌肉损伤中，天然组织无法修复和重塑创伤。在相同情况下，干细胞疗法在肌肉修复不足的情况下可促进再生，但主要问题似乎是注入的细胞在确切位置缺乏附着和存活的能力。在本研究中，对壳聚糖/聚乙烯醇纳米纤维支架进行了研究，以促进细胞附着并在再生过程中提供机械支持。使用扫描电子显微镜、X射线衍射和拉伸试验对支架进行了表征。研究了支架20天的降解和溶胀行为。通过MTT法对细胞-支架相互作用进行了10天的表征，并评估了支架在兔模型中的体内生物相容性。结果表明，细胞在支架上具有良好的活力、粘附、生长和铺展能力，这使得这种材料成为一种理想的工程肌肉移植物。©2016威利期刊公司。《生物医学材料研究杂志》A部分：第A104卷，第1720 - 1727页，2016年。
