Gott Matthew D, DeGraffenreid Anthony J, Feng Yutian, Phipps Michael D, Wycoff Donald E, Embree Mary F, Cutler Cathy S, Ketring Alan R, Jurisson Silvia S
Department of Chemistry, University of Missouri, Columbia, MO 65211, United States.
University of Missouri Research Reactor Center, Columbia, MO 65211, United States.
J Chromatogr A. 2016 Apr 8;1441:68-74. doi: 10.1016/j.chroma.2016.02.074. Epub 2016 Mar 2.
A simple column chromatographic method was developed to isolate (77)As (94±6% (EtOH/HCl); 74±11 (MeOH)) from germanium for potential use in radioimmunotherapy. The separation of arsenic from germanium was based on their relative affinities for different chromatographic materials in aqueous and organic environments. Using an organic or mixed mobile phase, germanium was selectively retained on a silica gel column as germanate, while arsenic was eluted from the column as arsenate. Subsequently, enriched (76)Ge (98±2) was recovered for reuse by elution with aqueous solution (neutral to basic). Greater than 98% radiolabeling yield of a (77)As-trithiol was observed from methanol separated [(77)As]arsenate [17].
开发了一种简单的柱色谱方法，用于从锗中分离出（77）As（乙醇/盐酸中为94±6%；甲醇中为74±11），以用于放射免疫治疗。砷与锗的分离基于它们在水性和有机环境中对不同色谱材料的相对亲和力。使用有机或混合流动相时，锗以锗酸盐的形式选择性地保留在硅胶柱上，而砷以砷酸盐的形式从柱上洗脱下来。随后，通过用中性至碱性的水溶液洗脱回收富集的（76）Ge（98±2）以供再利用。从甲醇分离得到的[（77）As]砷酸盐中观察到（77）As-三硫醇的放射性标记产率大于98%[17]。
