Chan W S, MacRobert A J, Phillips D, Hart I R
Photochem Photobiol. 1989 Nov;50(5):617-24. doi: 10.1111/j.1751-1097.1989.tb04317.x.
A highly sensitive charge-coupled device (CCD) camera, linked to a computerized image processor, has been used to obtain fluorescence images of chloro-aluminum sulfonated phthalocyanine (ClAlSPc), and its mono- to tetra-sulfonated constituents, in cells recovered from the peritoneal cavity of dye-treated mice. Immunofluorescence and cytochemistry showed that cells which preferentially took up ClAlSPc and its sulfonated species were predominantly of the monocyte-macrophage series. While this technique may be of use in determining the nature of those cells capable of taking up light sensitive dyes and, perhaps, the intracellular location of such agents, the possibility that photoinactivation by dye aggregation could reduce the sensitivity of this procedure is discussed.
一台与计算机图像处理器相连的高灵敏度电荷耦合器件（CCD）相机，已被用于获取从经染料处理的小鼠腹腔回收的细胞中氯铝磺化酞菁（ClAlSPc）及其单磺化至四磺化成分的荧光图像。免疫荧光和细胞化学表明，优先摄取ClAlSPc及其磺化产物的细胞主要是单核细胞-巨噬细胞系列。虽然这项技术可能有助于确定能够摄取光敏染料的细胞的性质，以及此类物质在细胞内的位置，但也讨论了染料聚集导致的光灭活可能降低该程序灵敏度的可能性。
