Ergüven S, Calgüner M, Gülmezoğlu E, Sungur C, Ozcebe O I
Hacettepe Universitesi, Tip Fakültesi, Mikrobiyoloji Anabilim Dali, Ankara.
Mikrobiyol Bul. 1989 Jan;23(1):1-11.
In this study, ELISA and VDRL tests have been employed in order to detect ACL antibodies in sera samples obtained from 105 patients with SLE, RA, scleroderma, dermatomyositis, primary Sjögren's syndrome, vasculitis and pulmonary thromboemboli; Using by IFAT, AMA-type 5 and other autoantibodies, RIA anti-DNA antibodies were also tested in SLE patients. In sera obtained from the patients with RA; autoantibodies and RF have been investigated. In SLE cases, ACL antibodies were found to be positive in 55.5% of the patients. In RA cases the rate of ACL antibodies were found to be 51.7%. In SLE cases, the presence of IgG-ACL antibody was found to be in association with thrombosis and fetal loss; while IgM-ACL antibody was found to be in association with fetal loss only. Serologic parameters such as VDRL and AMA type 5 were significantly connected with the presence of ACL-IgG and IgM antibodies (p less than 0.05). In cases of RA connection was detected only between IgG-ACL antibody and thrombosis. Concerning the other rheumatic autoimmune diseases; ACL antibodies were detected in cases: 4 patients out of 11 scleroderma, 1 patient out of 7 vasculitis, 2 patients out of 5 primary Sjögren's syndrome, and 3 patients out of 6 pulmonary thromboemboli.
在本研究中，采用酶联免疫吸附测定（ELISA）和性病研究实验室玻片试验（VDRL）检测从105例系统性红斑狼疮（SLE）、类风湿关节炎（RA）、硬皮病、皮肌炎、原发性干燥综合征、血管炎和肺血栓栓塞症患者获取的血清样本中的抗心磷脂（ACL）抗体；还通过间接免疫荧光法（IFAT）检测5型抗线粒体抗体（AMA-type 5）和其他自身抗体，对SLE患者检测放射免疫分析法（RIA）抗DNA抗体。在RA患者获取的血清中，研究了自身抗体和类风湿因子（RF）。在SLE病例中，发现55.5%的患者ACL抗体呈阳性。在RA病例中，ACL抗体的阳性率为51.7%。在SLE病例中，发现IgG-ACL抗体的存在与血栓形成和流产相关；而IgM-ACL抗体仅与流产相关。血清学参数如VDRL和5型AMA与ACL-IgG和IgM抗体的存在显著相关（p小于0.05）。在RA病例中，仅在IgG-ACL抗体与血栓形成之间检测到关联。关于其他风湿性自身免疫性疾病，在以下病例中检测到ACL抗体：11例硬皮病患者中有4例，7例血管炎患者中有1例，5例原发性干燥综合征患者中有2例，6例肺血栓栓塞症患者中有3例。
