Gripenberg M, Helve T, Kurki P
J Rheumatol. 1985 Oct;12(5):934-9.
The occurrence of antibodies against the total histone complex and the histone fraction H1, antibodies against denatured (ss) DNA and the synthetic double stranded polynucleotide poly dAT, as well as rheumatoid factors (RF) was determined in patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS) using enzyme linked immunosorbent assays (ELISA). Antihistone antibodies could be demonstrated at a frequency of about 17% in the patients with systemic rheumatic disease with no differences between the groups, even if there was a tendency for anti-H1 antibodies to occur more often in the SLE and SS patients than in the RA patients. Some of the antihistone antibody activity seen in the RA patients seems to be due to crossreactive RF. All patient groups showed significant IgG anti-ssDNA antibody activity compared to the controls, but the highest antibody levels were seen in the SLE patients. IgG antipoly dAT antibodies occurred significantly more often and at higher levels in the SLE patients than in the other patient groups. Although the individual tests did not readily distinguish the 3 diseases from each other, the antibody profiles were different. Patients with SS had the broadest reactivity, and the SLE patients had antibodies predominantly restricted to polynucleotides.
采用酶联免疫吸附测定法（ELISA），测定了类风湿关节炎（RA）、系统性红斑狼疮（SLE）和干燥综合征（SS）患者体内针对总组蛋白复合物和组蛋白H1组分的抗体、针对变性（单链）DNA和合成双链多聚核苷酸聚dAT的抗体以及类风湿因子（RF）。在患有全身性风湿性疾病的患者中，抗组蛋白抗体的检出频率约为17%，各疾病组之间无差异，尽管SLE和SS患者中抗H1抗体的出现频率有高于RA患者的趋势。RA患者中出现的一些抗组蛋白抗体活性似乎是由于交叉反应性RF所致。与对照组相比，所有患者组均显示出显著的IgG抗单链DNA抗体活性，但SLE患者的抗体水平最高。IgG抗聚dAT抗体在SLE患者中的出现频率显著高于其他患者组，且水平更高。尽管单项检测不易区分这三种疾病，但抗体谱有所不同。SS患者的反应性最广泛，而SLE患者的抗体主要局限于多聚核苷酸。
