虎杖及其内生放线菌链霉菌属A0916乙酸乙酯提取物的化学成分、抗菌和抗氧化活性比较分析

Comparative analysis of chemical constituents, antimicrobial and antioxidant activities of ethylacetate extracts of Polygonum cuspidatum and its endophytic actinomycete, Streptomyces sp. A0916.

作者信息

Wang Lei, Qiu Peng, Long Xiu-Feng, Zhang Shuai, Zeng Zhi-Gang, Tian Yong-Qiang

机构信息

Key laboratory of Leather Chemistry and engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, China; Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China.

Key laboratory of Leather Chemistry and engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, China.

出版信息

Chin J Nat Med. 2016 Feb;14(2):117-123. doi: 10.1016/S1875-5364(16)60004-3.

DOI:10.1016/S1875-5364(16)60004-3

PMID:26968677

Abstract

The present study investigated the chemical composition of ethylacetate extracts from an endophytic actinomycete Streptomyces sp. A0916 and its host Polygonum cuspidatum. A comparative analysis of the antimicrobial and antioxidant properties of the extracts was also conducted. 32 compounds of P. cuspidatum and 23 compounds of Streptomyces sp. A0916 were isolated and identified by GC/MS. Antimicrobial activities of the extracts were evaluated using eight microbial strains (3 Gram-positive bacteria, 3 Gram-negative bacteria, and 2 fungi). The Streptomyces sp. A0916 extracts showed a wide range of antimicrobial activities and presented greater antimicrobial effectiveness than the P. cuspidatum extracts. The minimum inhibitory concentration (MIC) of Streptomyces sp. A0916 extracts against the ampicillin-resistant strain Enterococcus faecium SIIA843 was 32 μg·mL(-1). Furthermore, the extracts had greater antimicrobial effect against Gram-positive bacteria than Gram-negative bacteria. Finally, the antioxidant activity of the Streptomyces sp. A0916 extracts was equal to that of the P. cuspidatum extracts. In conclusion, our results suggest that the endophytic actinomycetes of the medicinal plants are an important source of bioactive substances.

摘要

本研究调查了内生放线菌链霉菌属A0916及其宿主虎杖的乙酸乙酯提取物的化学成分。还对提取物的抗菌和抗氧化性能进行了比较分析。通过气相色谱/质谱联用仪分离并鉴定了虎杖的32种化合物和链霉菌属A0916的23种化合物。使用8种微生物菌株（3种革兰氏阳性菌、3种革兰氏阴性菌和2种真菌）评估提取物的抗菌活性。链霉菌属A0916提取物显示出广泛的抗菌活性，并且比虎杖提取物具有更高的抗菌效力。链霉菌属A0916提取物对耐氨苄青霉素的粪肠球菌SIIA843的最低抑菌浓度（MIC）为32μg·mL⁻¹。此外，提取物对革兰氏阳性菌的抗菌效果比对革兰氏阴性菌的抗菌效果更好。最后，链霉菌属A0916提取物的抗氧化活性与虎杖提取物的抗氧化活性相当。总之，我们的结果表明药用植物的内生放线菌是生物活性物质的重要来源。