Panayotov Ivan V, Végh Attila G, Martin Marta, Vladimirov Boyan, Larroque Christian, Gergely Csilla, Cuisinier Frédéric J G, Estephan Elias
LBN, University Montpellier, Montpellier, France.
CSERD, CHU Montpellier, Montpellier, France.
Front Bioeng Biotechnol. 2023 Jun 20;11:1165853. doi: 10.3389/fbioe.2023.1165853. eCollection 2023.
The functionalization of titanium (Ti) and titanium alloys (Ti6Al4V) implant surfaces via material-specific peptides influence host/biomaterial interaction. The impact of using peptides as molecular linkers between cells and implant material to improve keratinocyte adhesion is reported. The metal binding peptides (MBP-1, MBP-2) SVSVGMKPSPRP and WDPPTLKRPVSP were selected via phage display and combined with laminin-5 or E-cadherin epithelial cell specific peptides (CSP-1, CSP-2) to engineer four metal-cell specific peptides (MCSPs). Single-cell force spectroscopy and cell adhesion experiments were performed to select the most promising candidate. tests using the dental implant for rats showed that the selected bi functional peptide not only enabled stable cell adhesion on the trans-gingival part of the dental implant but also arrested the unwanted apical migration of epithelial cells. The results demonstrated the outstanding performance of the bioengineered peptide in improving epithelial adhesion to Ti based implants and pointed towards promising new opportunities for applications in clinical practice.
通过特定材料的肽对钛（Ti）及钛合金（Ti6Al4V）植入物表面进行功能化处理会影响宿主/生物材料的相互作用。本文报道了使用肽作为细胞与植入材料之间的分子连接体来改善角质形成细胞黏附的影响。通过噬菌体展示筛选出金属结合肽（MBP - 1、MBP - 2）SVSVGMKPSPRP和WDPPTLKRPVSP，并将其与层粘连蛋白 - 5或E - 钙黏蛋白上皮细胞特异性肽（CSP - 1、CSP - 2）相结合，设计出四种金属 - 细胞特异性肽（MCSP）。进行单细胞力谱分析和细胞黏附实验以筛选出最有前景的候选肽。对大鼠牙种植体的测试表明，所选的双功能肽不仅能使细胞在牙种植体的龈上部分实现稳定黏附，还能阻止上皮细胞不必要的根尖迁移。结果证明了这种生物工程肽在改善上皮细胞与钛基植入物黏附方面的卓越性能，并为临床实践中的应用指明了充满希望的新机遇。
