Kammann U, Akcha F, Budzinski H, Burgeot T, Gubbins M J, Lang T, Le Menach K, Vethaak A D, Hylland K
Thünen Institut of Fisheries Ecology, Palmaille 9, D-22767, Hamburg & Deichstr. 12, 27472 Cuxhaven, Germany.
Ifremer, Department of Biogeochemistry and Ecotoxicology, BP 21105, 44311 Nantes Cedex 03, France.
Mar Environ Res. 2017 Mar;124:41-45. doi: 10.1016/j.marenvres.2016.02.014. Epub 2016 Mar 3.
Polycyclic aromatic hydrocarbons (PAH) are environmental contaminants that pose significant risk to health of fish. The International Workshop on Integrated Assessment of Contaminant Impacts on the North Sea (ICON) provided the framework to investigate biomarker responses as well as contaminant concentrations side by side in marine ecosystems. Concentrations of the main PAH metabolites 1-hydroxypyrene, 1-hydroxyphenanthren and 3-hydroxybenzo(a)pyrene were determined in bile by HPLC with fluorescence detection. Fish species under investigation were dab (Limanda limanda), flounder (Platichthys flesus) and haddock (Melanogrammus aeglefinus). A contamination gradient was demonstrated from the low contaminated waters of Iceland and off-shore regions of the North Sea towards higher concentrations in coastal areas. Concentrations of PAH metabolites differed primarily according to sampling region and secondarily to species.
多环芳烃（PAH）是对鱼类健康构成重大风险的环境污染物。北海污染物影响综合评估国际研讨会（ICON）提供了一个框架，用于在海洋生态系统中同时研究生物标志物反应和污染物浓度。通过高效液相色谱-荧光检测法测定胆汁中主要PAH代谢物1-羟基芘、1-羟基菲和3-羟基苯并(a)芘的浓度。所调查的鱼类物种有鲽鱼（欧洲黄盖鲽）、比目鱼（欧洲鳎）和黑线鳕。从冰岛低污染水域和北海近海区域到沿海地区较高浓度，呈现出一个污染梯度。PAH代谢物的浓度主要根据采样区域而有所不同，其次根据物种而有所不同。
