The NIH Big Data to Knowledge (BD2K) Center of Excellence in Biomedical Computing at UCLA, Los Angeles, California 90095, USA.
Department of Physiology, University of California at Los Angeles, Los Angeles, California 90095, USA.
Sci Data. 2016 Mar 15;3:160015. doi: 10.1038/sdata.2016.15.
Protein stability is a major regulatory principle of protein function and cellular homeostasis. Despite limited understanding on mechanisms, disruption of protein turnover is widely implicated in diverse pathologies from heart failure to neurodegenerations. Information on global protein dynamics therefore has the potential to expand the depth and scope of disease phenotyping and therapeutic strategies. Using an integrated platform of metabolic labeling, high-resolution mass spectrometry and computational analysis, we report here a comprehensive dataset of the in vivo half-life of 3,228 and the expression of 8,064 cardiac proteins, quantified under healthy and hypertrophic conditions across six mouse genetic strains commonly employed in biomedical research. We anticipate these data will aid in understanding key mitochondrial and metabolic pathways in heart diseases, and further serve as a reference for methodology development in dynamics studies in multiple organ systems.
蛋白质稳定性是蛋白质功能和细胞内稳态的主要调节原则。尽管对其机制的了解有限，但蛋白质周转率的破坏广泛涉及从心力衰竭到神经退行性疾病等多种病理学。因此，有关全局蛋白质动态的信息有可能扩展疾病表型和治疗策略的深度和范围。我们使用代谢标记、高分辨率质谱和计算分析的综合平台，报告了在六种常用于生物医学研究的小鼠遗传品系中，在健康和肥大条件下，3228 种体内半衰期和 8064 种心脏蛋白表达的综合数据集。我们预计这些数据将有助于理解心脏病中线粒体和代谢途径的关键，并且可以进一步作为多个器官系统动力学研究中方法开发的参考。
