Ameen Fuad, Moslem Mohamed, Hadi Sarfaraz, Al-Sabri Ahmed E
Department of Botany & Microbiology, Faculty of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Saudi J Biol Sci. 2016 Mar;23(2):211-8. doi: 10.1016/j.sjbs.2015.04.005. Epub 2015 Apr 7.
Mangrove sediments were collected from major mangrove stands on the Red Sea Coast of Saudi Arabia. Forty five isolates belonging to 12 genera were purified and five isolates as well as their consortium were found to be able to grow in association with petroleum oil as sole carbon source under in vitro conditions. The isolated strains were identified based on internal transcribed spacer (ITS) rDNA sequence analysis. The fungal strains with the greatest potentiality to degrade diesel oil, without developing antagonistic activity, were identified as Alternaria alternata, Aspergillus terreus, Cladosporium sphaerospermum, Eupenicillium hirayamae and Paecilomyces variotii. As compared to the controls, these fungi accumulated significantly higher biomass, produced extracellular enzymes and liberated larger volumes of CO2. These observations with GC-MS data confirm that these isolates displayed rapid diesel oil bioremoval and when used together as a consortium, there was no antagonistic activity.
从沙特阿拉伯红海沿岸的主要红树林采集了红树林沉积物。纯化了属于12个属的45株分离物，发现5株分离物及其联合体能够在体外条件下以石油作为唯一碳源生长。基于内部转录间隔区（ITS）rDNA序列分析对分离菌株进行了鉴定。具有最大柴油降解潜力且无拮抗活性的真菌菌株被鉴定为链格孢、土曲霉、球形枝孢、平山青霉和拟青霉。与对照相比，这些真菌积累了显著更高的生物量，产生了胞外酶并释放了更多的二氧化碳。这些观察结果与气相色谱-质谱数据证实，这些分离物显示出快速的柴油生物去除能力，并且当作为联合体一起使用时，没有拮抗活性。
