Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Pharmaceutical Chemistry Department, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
PLoS One. 2021 Aug 26;16(8):e0256376. doi: 10.1371/journal.pone.0256376. eCollection 2021.
The use of potent fungal mixed cultures is a promising technique for the biodegradation of crude oil. Four isolates of fungi, namely, Alternaria alternata (AA-1), Aspergillus flavus (AF-3), Aspergillus terreus (AT-7), and Trichoderma harzianum (TH-5), were isolated from date palm soil in Saudi Arabia. The mixed fungal of the four isolates have a powerful tool for biodegradation up to 73.6% of crude oil (1%, w/v) in 14 days. The fungal consortium no. 15 containing the four isolates (1:1:1:1) performed significantly better as a biodegradation agent than other consortium in a variety of environmental factors containing crude oil concentration, incubation temperature, initial pH, biodegradation time and the salinity of the medium. The fungal consortium showed better performance in the biodegradation of normal alkanes (n-alkanes) than that of the polycyclic aromatic hydrocarbons (PAHs); the biodegradation efficiency of normal alkanes of the fungal consortium (67.1%) was clearly high than that of the PAHs (56.8%).
利用强效真菌混合培养物是生物降解原油的一种很有前途的技术。从沙特阿拉伯的枣椰树土壤中分离出四种真菌，分别为链格孢（AA-1）、黄曲霉（AF-3）、土曲霉（AT-7）和哈茨木霉（TH-5）。这四种真菌的混合培养物是一种强大的工具，可在 14 天内将原油（1%，w/v）生物降解高达 73.6%。含有这四种真菌的真菌混合物 15（1:1:1:1）在各种环境因素（包括原油浓度、培养温度、初始 pH 值、生物降解时间和培养基盐度）下作为生物降解剂的性能明显优于其他混合物。真菌混合物在生物降解正烷烃（直链烷烃）方面的性能优于多环芳烃（PAHs）；真菌混合物对正烷烃的生物降解效率（67.1%）明显高于对 PAHs 的生物降解效率（56.8%）。
