Lietman Steven A
Steven A Lietman, Cleveland Clinic Departments of BME and Orthopaedics, Cleveland Clinic Foundation, Cleveland, OH 44195, United States.
World J Orthop. 2016 Mar 18;7(3):149-55. doi: 10.5312/wjo.v7.i3.149.
Articular cartilage repair techniques are challenging. Human embryonic stem cells and induced pluripotent stem cells (iPSCs) theoretically provide an unlimited number of specialized cells which could be used in articular cartilage repair. However thus far chondrocytes from iPSCs have been created primarily by viral transfection and with the use of cocultured feeder cells. In addition chondrocytes derived from iPSCs have usually been formed in condensed cell bodies (resembling embryoid bodies) that then require dissolution with consequent substantial loss of cell viability and phenotype. All of these current techniques used to derive chondrocytes from iPSCs are problematic but solutions to these problems are on the horizon. These solutions will make iPSCs a viable alternative for articular cartilage repair in the near future.
关节软骨修复技术具有挑战性。人类胚胎干细胞和诱导多能干细胞（iPSC）理论上可提供无限数量的特定细胞，可用于关节软骨修复。然而，迄今为止，iPSC来源的软骨细胞主要通过病毒转染并使用共培养的饲养细胞来产生。此外，iPSC衍生的软骨细胞通常在浓缩的细胞体中形成（类似于胚状体），然后需要溶解，这会导致细胞活力和表型的大量丧失。目前所有用于从iPSC中获取软骨细胞的技术都存在问题，但这些问题的解决方案即将出现。这些解决方案将使iPSC在不久的将来成为关节软骨修复的可行替代方案。
