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基质辅助激光解吸电离飞行时间质谱法用于鉴定、类、和物种的性能。

Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of , -Like, , and Species.

作者信息

Wei Linwei, Shao Jin, Song Yinggai, Wan Zhe, Yao Limin, Wang Hong, Yu Jin

机构信息

Department of Dermatology and Venereology, Peking University First Hospital, Beijing, China.

Research Center for Medical Mycology, Peking University, Beijing, China.

出版信息

Front Microbiol. 2022 Mar 2;13:841286. doi: 10.3389/fmicb.2022.841286. eCollection 2022.

DOI:10.3389/fmicb.2022.841286
PMID:35308359
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8924672/
Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a powerful microorganism identification tool. Research on MALDI-TOF MS identification of rare filamentous fungi is still lacking. This study aimed to evaluate the performance of MALDI-TOF MS in the identification of , -like, , and species. Sabouraud broth cultivation and formic acid/acetonitrile protein extraction were used for MALDI-TOF MS identification by a Bruker Biotyper system. An in-house database containing 29 isolates of , -like, , and spp. was constructed. A total of 52 clinical isolates were identified using the Bruker Filamentous Fungi Library v1.0 (FFL v1.0) alone, and Filamentous Fungi Library v1.0 plus the in-house library, respectively. The mass spectrum profile (MSP) dendrograms of the 28 isolates, 26 -like isolates, and 27 and isolates were constructed by MALDI Biotyper OC 4.0 software, respectively. The correct species identification rate significantly improved when using the combined databases compared with that when using FFL v1.0 alone ( spp., 75% versus 0%; -like spp., 100% versus 0%; and spp., 100% versus 62.5%). The MSP dendrograms differentiated -like species, and species clearly, but cannot distinguish species in the complex. In conclusion, with an expanded database, MALDI-TOF MS is an effective tool for the identification of , -like, , and species.

摘要

基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）已成为一种强大的微生物鉴定工具。关于MALDI-TOF MS鉴定罕见丝状真菌的研究仍然缺乏。本研究旨在评估MALDI-TOF MS在鉴定[具体菌种1]、[具体菌种2]样、[具体菌种3]和[具体菌种4]菌种方面的性能。采用沙氏肉汤培养法和甲酸/乙腈蛋白提取法，通过布鲁克微生物鉴定系统进行MALDI-TOF MS鉴定。构建了一个包含29株[具体菌种1]、[具体菌种2]样、[具体菌种3]和[具体菌种4]菌株的内部数据库。分别使用布鲁克丝状真菌文库v1.0（FFL v1.0）以及FFL v1.0加内部文库对总共52株临床分离株进行鉴定。分别通过MALDI Biotyper OC 4.0软件构建了28株[具体菌种1]分离株、26株[具体菌种2]样分离株以及27株[具体菌种3]和[具体菌种4]分离株的质谱图谱（MSP）树状图。与单独使用FFL v1.0相比，使用联合数据库时正确的菌种鉴定率显著提高（[具体菌种1]，75%对0%；[具体菌种2]样，10对0%；[具体菌种3]和[具体菌种4]，100%对62.5%）。MSP树状图能够清晰地区分[具体菌种2]样菌种、[具体菌种3]和[具体菌种4]菌种，但无法区分[具体菌种1]复合群中的菌种。总之，通过扩展数据库，MALDI-TOF MS是鉴定[具体菌种1]、[具体菌种2]样、[具体菌种3]和[具体菌种4]菌种的有效工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/f51c0095dd94/fmicb-13-841286-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/039a82dadb9a/fmicb-13-841286-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/3c7e71c21139/fmicb-13-841286-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/42d805a9e10a/fmicb-13-841286-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/eed1bf1f5704/fmicb-13-841286-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/79f24dd6a5b4/fmicb-13-841286-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/00ac2244d2de/fmicb-13-841286-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/f51c0095dd94/fmicb-13-841286-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/039a82dadb9a/fmicb-13-841286-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/3c7e71c21139/fmicb-13-841286-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/42d805a9e10a/fmicb-13-841286-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/eed1bf1f5704/fmicb-13-841286-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/79f24dd6a5b4/fmicb-13-841286-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/00ac2244d2de/fmicb-13-841286-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c99/8924672/f51c0095dd94/fmicb-13-841286-g007.jpg

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