用于检测产碳青霉烯酶肠杆菌科细菌的Xpert Carba - R试剂盒的改进
Improvement of the Xpert Carba-R Kit for the Detection of Carbapenemase-Producing Enterobacteriaceae.
作者信息
Dortet Laurent, Fusaro Mathieu, Naas Thierry
机构信息
Bacteriology-Hygiene Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France EA7361 "Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases," Paris-Sud University, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicêtre, France Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France Joint Research Unit EERA "Evolution and Ecology of Resistance to Antibiotics," Institut Pasteur-APHP-Université Paris-Sud, Paris, France.
Bacteriology-Hygiene Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France.
出版信息
Antimicrob Agents Chemother. 2016 May 23;60(6):3832-7. doi: 10.1128/AAC.00517-16. Print 2016 Jun.
Abstract
The Xpert Carba-R kit, version 2 (v2), which has been improved for the efficient detection of blaOXA-181 and blaOXA-232 genes, was tested on a collection of 150 well-characterized enterobacterial isolates that had a reduced susceptibility to carbapenems. The performance of the Xpert Carba-R v2 was high, as it was able to detect the five major carbapenemases (NDM, VIM, IMP, KPC, and OXA-48). Thus, it is now well adapted to the carbapenemase-producing Enterobacteriaceae epidemiology of many countries worldwide.
摘要
已改进用于高效检测blaOXA - 181和blaOXA - 232基因的Xpert Carba - R试剂盒版本2（v2），在150株对碳青霉烯类药物敏感性降低的、特征明确的肠杆菌科分离株样本上进行了测试。Xpert Carba - R v2的性能很高，因为它能够检测出五种主要的碳青霉烯酶（NDM、VIM、IMP、KPC和OXA - 48）。因此，它现在非常适合全球许多国家产碳青霉烯酶肠杆菌科细菌的流行病学情况。
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