Pickens C Austin, Matsuo Karen H, Fenton Jenifer I
Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI, 48824, United States of America.
PLoS One. 2016 Mar 29;11(3):e0149305. doi: 10.1371/journal.pone.0149305. eCollection 2016.
Obesity, in particular abdominal obesity, alters the composition of plasma and tissue fatty acids (FAs), which contributes to inflammation and insulin resistance. FA metabolism is modulated by desaturases and may affect adipokine and insulin secretion. Therefore, we examined relationships between adipokines, a marker of insulin production, and plasma FA desaturase enzyme activity estimates (EAEs) in obesity. Plasma phospholipid (PPL) FAs were isolated from 126 males (ages 48 to 65 years), derivatized, and analyzed using gas chromatography. Delta-6 desaturase (D6D) and delta-5 desaturase (D5D) EAEs were calculated as the ratio of PPL 20:3/18:2 and 20:4/20:3, respectively. In body mass index (BMI) and waist circumference (WC) adjusted polytomous logistic regression analyses, PPL FAs and FA desaturase EAEs were associated with C-peptide and adiponectin. Individuals with elevated D6D EAEs were less likely (OR 0.33) to have serum adiponectin concentrations > 5.37 μg/mL, compared with adiponectin concentrations ≤ 3.62 μg/mL. Individuals with increased D5D EAEs were less likely (OR 0.8) to have C-peptide concentrations ≥ 3.32 ng/mL, and > 1.80 and ≤ 3.29 ng/mL, compared with those with C-peptide ≤ 1.76 ng/mL. The proinflammatory cytokine tumor necrosis factor-α (TNF-α) was positively associated with C-peptide, but TNF-α was not associated with the D5D EAE. C-peptide and adiponectin concentrations are associated with specific PPL FAs and FA desaturase EAEs. The relationship between C-peptide concentrations and D5D EAEs remained significant after adjusting for BMI, WC, and TNF-α. Thus, future research should investigate whether D5D inhibition may occur through a C-peptide mediated pathway.
肥胖，尤其是腹部肥胖，会改变血浆和组织脂肪酸（FAs）的组成，这会导致炎症和胰岛素抵抗。脂肪酸代谢受去饱和酶调节，并可能影响脂肪因子和胰岛素分泌。因此，我们研究了肥胖患者中脂肪因子（胰岛素产生的标志物）与血浆脂肪酸去饱和酶酶活性估计值（EAEs）之间的关系。从126名男性（年龄48至65岁）中分离出血浆磷脂（PPL）脂肪酸，进行衍生化处理，并使用气相色谱法进行分析。分别将Δ-6去饱和酶（D6D）和Δ-5去饱和酶（D5D）的EAEs计算为PPL 20:3/18:2和20:4/20:3的比值。在调整了体重指数（BMI）和腰围（WC）的多分类逻辑回归分析中，PPL脂肪酸和脂肪酸去饱和酶EAEs与C肽和脂联素相关。与脂联素浓度≤3.62μg/mL相比，D6D EAEs升高的个体血清脂联素浓度>5.37μg/mL的可能性较小（OR 0.33）。与C肽≤1.76 ng/mL的个体相比，D5D EAEs升高的个体C肽浓度≥3.32 ng/mL以及>1.80且≤3.29 ng/mL的可能性较小（OR 0.8）。促炎细胞因子肿瘤坏死因子-α（TNF-α）与C肽呈正相关，但TNF-α与D5D EAE无关。C肽和脂联素浓度与特定的PPL脂肪酸和脂肪酸去饱和酶EAEs相关。在调整了BMI、WC和TNF-α后，C肽浓度与D5D EAEs之间的关系仍然显著。因此，未来的研究应调查D5D抑制是否可能通过C肽介导的途径发生。
