Department of Clinical Pharmacology, Medical University of Vienna, Austria.
Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Austria.
Transl Vis Sci Technol. 2020 Jun 25;9(7):34. doi: 10.1167/tvst.9.7.34. eCollection 2020 Jun.
To investigate the repeatability and reproducibility of total retinal blood flow measurements using a custom-built dual-beam bidirectional Doppler optical coherence tomography (OCT) system in healthy subjects.
Repeatability and reproducibility were analyzed in 10 and 34 healthy subjects, respectively. For repeatability, measurements were taken twice within 30 minutes, for reproducibility, twice within two to five weeks. Two analysis approaches were compared for calculation of absolute blood velocities: a previously published approach resulting in values for total arterial (Q) and total venous blood flow (Q) and a novel approach taking into account that there is a fixed relation between the phase shift in the two OCT channels (Q Q). Repeatability and reproducibility were quantified using intraclass correlation coefficients (ICCs).
For Q and Q, ICC values between 0.78 and 0.84 were obtained. Q and Q values revealed better repeatability and reproducibility as compared to the convential appoach. Repeatability ICCs for Q and Q were between 0.91 and 0.93, and reproducibility ICCs were between 0.87 and 0.91 indicating excellent reproducibility. Good agreement was observed between total retinal blood flow values as measured from retinal arteries and retinal veins.
Measurement of total retinal blood flow using dual-beam Doppler OCT shows excellent reproducibility, which can further be improved by using a novel algorithm for calculating blood velocities in retinal vessels.
Our data indicate that dual-beam Doppler OCT can be used for longitudinal studies. Hence, quantitative retinal blood flow may be established as a biomarker for progression vascular eye diseases.
使用定制的双光束双向多普勒光相干断层扫描（OCT）系统在健康受试者中研究总视网膜血流测量的可重复性和再现性。
分别对 10 名和 34 名健康受试者进行了可重复性和再现性分析。对于可重复性，在 30 分钟内进行了两次测量，对于再现性，在两到五周内进行了两次测量。为了计算绝对血流速度，比较了两种分析方法：一种是先前发表的方法，该方法产生总动脉（Q）和总静脉血流（Q）的值，另一种是考虑到两个 OCT 通道之间存在固定相位差的新方法（Q-Q）。使用组内相关系数（ICC）来量化可重复性和再现性。
对于 Q 和 Q，获得了 0.78 到 0.84 之间的 ICC 值。与传统方法相比，Q 和 Q 值的可重复性和再现性更好。Q 和 Q 的可重复性 ICC 值在 0.91 到 0.93 之间，再现性 ICC 值在 0.87 到 0.91 之间，表明具有出色的再现性。从视网膜动脉和视网膜静脉测量的总视网膜血流值之间观察到良好的一致性。
使用双光束多普勒 OCT 测量总视网膜血流具有出色的可再现性，通过使用计算视网膜血管血流速度的新算法可以进一步提高可再现性。
杨硕
