Paul Sudip, Islam Md Aminul, Tanvir E M, Ahmed Romana, Das Sagarika, Rumpa Nur-E-Noushin, Hossen Md Sakib, Parvez Mashud, Gan Siew Hua, Khalil Md Ibrahim
Laboratory of Preventive and Integrative Biomedicine, Department of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka 1342, Bangladesh.
Laboratory of Preventive and Integrative Biomedicine, Department of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka 1342, Bangladesh; Department of Biochemistry and Molecular Biology, Gono University, Mirzanagar, Savar, Dhaka 1344, Bangladesh.
Evid Based Complement Alternat Med. 2016;2016:9470954. doi: 10.1155/2016/9470954. Epub 2016 Feb 29.
Although Citrus macroptera (Rutaceae), an indigenous fruit in Bangladesh, has long been used in folk medicine, however, there is a lack of information concerning its protective effects against oxidative damage. The protective effects of an ethanol extract of Citrus macroptera (EECM) against acetaminophen-induced hepatotoxicity and nephrotoxicity were investigated in rats. Rats (treatment groups) were pretreated with EECM at doses of 250, 500, and 1000 mg/kg, respectively, orally for 30 days followed by acetaminophen administration. Silymarin (100 mg/kg) was administered as a standard drug over a similar treatment period. Our findings indicated that oral administration of acetaminophen induced severe hepatic and renal injuries associated with oxidative stress, as observed by 2-fold higher lipid peroxidation (TBARS) compared to control. Pretreatment with EECM prior to acetaminophen administration significantly improved all investigated biochemical parameters, that is, transaminase activities, alkaline phosphatase, lactate dehydrogenase, γ-glutamyl transferase activities and total bilirubin, total cholesterol, triglyceride and creatinine, urea, uric acid, sodium, potassium and chloride ions, and TBARS levels. These findings were confirmed by histopathological examinations. The improvement was prominent in the group that received 1000 mg/kg EECM. These findings suggested that C. macroptera fruit could protect against acetaminophen-induced hepatonephrotoxicity, which might be via the inhibition of lipid peroxidation.
尽管孟加拉国本土水果大翼柑橘（芸香科）长期以来一直用于民间医学，但关于其对氧化损伤的保护作用却缺乏相关信息。本研究在大鼠中探究了大翼柑橘乙醇提取物（EECM）对醋氨酚诱导的肝毒性和肾毒性的保护作用。大鼠（治疗组）分别以250、500和1000mg/kg的剂量口服EECM预处理30天，随后给予醋氨酚。水飞蓟宾（100mg/kg）作为标准药物在相似的治疗期内给药。我们的研究结果表明，口服醋氨酚会导致严重的肝损伤和肾损伤，并伴有氧化应激，与对照组相比，脂质过氧化（TBARS）水平高出2倍。在给予醋氨酚之前用EECM预处理可显著改善所有检测的生化参数，即转氨酶活性、碱性磷酸酶、乳酸脱氢酶、γ-谷氨酰转移酶活性以及总胆红素、总胆固醇、甘油三酯和肌酐、尿素、尿酸、钠、钾和氯离子以及TBARS水平。这些结果通过组织病理学检查得到证实。在接受1000mg/kg EECM的组中改善最为显著。这些结果表明，大翼柑橘果实可以预防醋氨酚诱导的肝肾毒性，这可能是通过抑制脂质过氧化实现的。
