Graham Jay P, Vasco Karla, Trueba Gabriel
Milken Institute School of Public Health, George Washington University, NW, Washington, DC, USA.
Microbiology Institute, Universidad San Francisco de Quit, Diego de Robles y Vía Interoceánica, Quito, Ecuador.
Environ Microbiol Rep. 2016 Jun;8(3):382-7. doi: 10.1111/1758-2229.12396. Epub 2016 May 11.
Domestic animals and animal products are the source of pathogenic Campylobacter jejuni and C. coli in industrialized countries, yet little is known about the transmission of these bacteria in developing countries. Guinea pigs (Cavia porcellus) are commonly raised for food in the Andean region of South America, however, limited research has characterized this rodent as a reservoir of zoonotic enteric pathogens. In this study, we examined the prevalence of Campylobacter spp. in 203 fecal samples from domestic animals of 59 households in a semi-rural parish of Quito, Ecuador. Of the twelve animal species studied, guinea pigs showed the highest prevalence of C. jejuni (n = 39/40; 97.5%). Multilocus sequence typing (MLST) was used to characterize the genetic relationship of C. jejuni from domestic animals and 21 sequence types (STs) were identified. The majority of STs from guinea pigs appeared to form new clonal complexes that were not related to STs of C. jejuni isolated from other animal species and shared only a few alleles with other C. jejuni previously characterized. The study identifies guinea pigs as a major reservoir of C. jejuni and suggests that some C. jejuni strains are adapted to this animal species.
在工业化国家，家畜及其产品是致病性空肠弯曲菌和结肠弯曲菌的来源，但在发展中国家，关于这些细菌的传播情况却知之甚少。豚鼠（Cavia porcellus）在南美洲安第斯地区通常作为食物饲养，然而，仅有有限的研究将这种啮齿动物描述为人畜共患肠道病原体的宿主。在本研究中，我们检测了厄瓜多尔基多一个半乡村教区59户家庭家畜的203份粪便样本中空肠弯曲菌属的流行情况。在所研究的12种动物中，豚鼠的空肠弯曲菌流行率最高（n = 39/40；97.5%）。多位点序列分型（MLST）用于鉴定家畜中空肠弯曲菌的遗传关系，并确定了21种序列类型（STs）。豚鼠的大多数STs似乎形成了新的克隆复合体，与从其他动物物种分离的空肠弯曲菌的STs无关，并且仅与先前鉴定的其他空肠弯曲菌共享少数等位基因。该研究将豚鼠鉴定为空肠弯曲菌的主要宿主，并表明一些空肠弯曲菌菌株适应了这种动物物种。
