el Nahas S M, de Hondt H A, Abdou H E
Unit of Cell Biology, National Research Centre, Cairo, Egypt.
Mutat Res. 1989 Apr;222(4):409-14. doi: 10.1016/0165-1218(89)90116-x.
Curacron is an organophosphorus pesticide widely used in cotton fields. In order to assay its mutagenic potential in mammalian germ cells chromosomal aberrations in spermatogonial cells and sperm abnormalities were examined in mice after Curacron treatment. For studying chromosomal aberrations mice were treated both acutely (single treatment) and subacutely (for 5 consecutive days) with 3 dose levels of Curacron, 12, 36 and 72 mg/kg. Curacron was found to produce a significant increase in structural chromosomal aberrations after acute and subacute treatments. This increase was dose-dependent. A dose-dependent inhibition in mitotic activity in spermatogonia was also found. For studying sperm abnormalities mice were treated for 5 consecutive days with 20, 40 and 60 mg/kg. Morphological sperm abnormalities increased significantly after treatment with Curacron. The increase was dose-dependent. An inhibition of 40.2% in sperm count and of 74.5% in sperm motility occurred after treatment with 60 mg/kg Curacron. These results show that Curacron has a damaging effect on spermatogonial cells as well as on sperm morphology.
谷硫磷是一种广泛应用于棉田的有机磷农药。为了测定其对哺乳动物生殖细胞的诱变潜力，在给予谷硫磷处理后，检测了小鼠精原细胞的染色体畸变和精子异常情况。为研究染色体畸变，用12、36和72毫克/千克这3个剂量水平的谷硫磷对小鼠进行急性（单次处理）和亚急性（连续5天）处理。结果发现，急性和亚急性处理后，谷硫磷均使染色体结构畸变显著增加，且这种增加呈剂量依赖性。同时还发现，谷硫磷对精原细胞的有丝分裂活性有剂量依赖性抑制作用。为研究精子异常情况，用20、40和60毫克/千克的谷硫磷对小鼠连续处理5天。经谷硫磷处理后，精子形态异常显著增加，且这种增加呈剂量依赖性。用60毫克/千克谷硫磷处理后，精子数量抑制率为40.2%，精子活力抑制率为74.5%。这些结果表明，谷硫磷对精原细胞以及精子形态均有损害作用。
