Isik Hatice, Sahbaz Ahmet, Timur Hakan, Aynioglu Oner, Atalay Mert Sule, Balık Ahmet Rıfat, Erel Ozcan, Harma Muge
Department of Obstetrics and Gynecology, Bulent Ecevıt Unıversıty School of Medicine, Zonguldak, Turkey.
Gynecol Obstet Invest. 2017;82(2):113-118. doi: 10.1159/000445745. Epub 2016 Apr 12.
The study aimed to compare the dynamic thiol/disulfide homeostasis between patients with premature ovarian failure (POF) and healthy women. A total of 77 women, 40 POF and 37 healthy controls, were recruited from a university hospital between December 2013 and June 2015. Blood samples were taken from patients to evaluate follicle stimulating hormone (FSH), luteinizing hormone, estradiol and thiol/disulfide levels. A new, fully automated method was used to measure plasma thiol, total thiol and disulfide levels. Disulfide levels, disulfide/native thiol and disulfide/total thiol ratios were significantly increased, native thiol/total thiol levels were significantly decreased in POF patients compared to controls (p < 0.001). FSH was negatively correlated with native thiol and native thiol/total thiol levels and positively with disulfide, disulfide/native thiol, and disulfide/total thiol levels. This is the first study demonstrating the thiol/disulfide homeostasis in women with POF and may help us understanding the pathophysiology.
该研究旨在比较卵巢早衰（POF）患者与健康女性之间动态的硫醇/二硫化物稳态。2013年12月至2015年6月期间，从一家大学医院招募了77名女性，其中40名POF患者和37名健康对照者。采集患者血样以评估促卵泡激素（FSH）、黄体生成素、雌二醇和硫醇/二硫化物水平。采用一种全新的全自动方法测量血浆硫醇、总硫醇和二硫化物水平。与对照组相比，POF患者的二硫化物水平、二硫化物/天然硫醇和二硫化物/总硫醇比率显著升高，天然硫醇/总硫醇水平显著降低（p < 0.001）。FSH与天然硫醇和天然硫醇/总硫醇水平呈负相关，与二硫化物、二硫化物/天然硫醇和二硫化物/总硫醇水平呈正相关。这是第一项证明POF女性硫醇/二硫化物稳态的研究，可能有助于我们理解其病理生理学。
