Yıldırım Engin, Türkler Can, Görkem Ümit, Şimşek Ömer Yavuz, Yılmaz Ercan, Aladağ Hülya
Malatya Turgut Özal University Faculty of Medicine, Department of Obstetrics and Gynecology, Malatya, Turkey
Erzincan Binali Yıldırım University Faculty of Medicine, Department of Obstetrics and Gynecology, Erzincan, Turkey
Turk J Obstet Gynecol. 2021 Dec 24;18(4):298-303. doi: 10.4274/tjod.galenos.2021.16132.
Endometrial hyperplasia (EH) is considered an endometrial cancer precursor. This study aimed to determine the role of oxidative stress and thiol groups with antioxidant properties in EH pathogenesis.
In our prospective case-control study, participants were washed with 5 mL of saline before the endometrial biopsy. Endometrial washing fluid was taken into microtubules, and thiol and disulfide levels were analyzed using the Ellman reagent.
A total of 108 patients were in the EH group and 84 patients in the control group. The total and native thiol levels were higher values in the control group (p<0.001, for both). Disulfide levels were higher in the EH group (p<0.001). Native/total thiol ratio and disulfide/total thiol ratio were higher in the EH group (p<0.001, for both). The analysis performed in the control group revealed a significant positive correlation between estradiol and disulfide levels (r=0.322, p=0.033). No significant correlation was found between estradiol and disulfide in the EH group.
Oxidative stress level was higher in the washing fluids of patients with EH and this stress plays a role in the EH etiology.
子宫内膜增生（EH）被认为是子宫内膜癌的前体。本研究旨在确定氧化应激和具有抗氧化特性的硫醇基团在EH发病机制中的作用。
在我们的前瞻性病例对照研究中，参与者在子宫内膜活检前用5毫升生理盐水冲洗。将子宫内膜冲洗液收集到微量管中，使用埃尔曼试剂分析硫醇和二硫键水平。
EH组共有108例患者，对照组有84例患者。对照组的总硫醇和天然硫醇水平较高（两者p<0.001）。EH组的二硫键水平较高（p<0.001）。EH组的天然/总硫醇比率和二硫键/总硫醇比率较高（两者p<0.001）。在对照组中进行的分析显示雌二醇与二硫键水平之间存在显著正相关（r=0.322，p=0.033）。在EH组中未发现雌二醇与二硫键之间存在显著相关性。
EH患者冲洗液中的氧化应激水平较高，这种应激在EH病因学中起作用。
