Wei Li, Mao Meiling, Liu Honggang
a Department of Pathology , Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology , Beijing , PR China ;
b Beijing Tsinghua Changgung Hospital , Beijing , PR China.
Acta Otolaryngol. 2016 Sep;136(9):923-32. doi: 10.3109/00016489.2016.1165862. Epub 2016 Apr 12.
The present study indicates that miR-21 is involved in progression from normal to pre-malignant laryngeal lesions (PLLs) and from PLLs to laryngeal squamous cell carcinoma (LSCC). Furthermore, normalized PCR results for miR-21 might be used to discriminate between normal and ordinary hyperplasia before the emergence of dysplasias and pre-malignant lesions with malignant potential.
To investigate a sensitive marker that contributes to progression from normal tissue to PLLs and from PLLs to LSCC.
In 116 PLLs and LSCC patients and 19 without dysplasia matched sets of tissue and plasma samples from Beijing Tongren Hospital, miR-21 was analysed by droplet digital PCR and quantitative real-time polymerase chain reaction based on paraffin-embedded tumour tissue and plasma.
Compared with controls, miR-21 levels in tissue and plasma were significantly higher for both PLL and LSCC groups (for both groups vs controls: p < 0.0001 for tissue; p < 0.05 for plasma). Areas under receiver-operating curves (AUC) for tissue miR-21 were PLL group vs controls: 0.9026 and LSCC group vs controls: 0.8681. For plasma miR-21, AUCs were PLL group vs controls: 0.7355 and LSCC group vs control: 0.6723.
本研究表明，miR-21参与了从正常喉组织到喉癌前病变（PLL）以及从PLL到喉鳞状细胞癌（LSCC）的进展过程。此外，miR-21的标准化PCR结果可能用于在发育异常和具有恶性潜能的癌前病变出现之前区分正常组织和普通增生。
研究一种有助于从正常组织进展到PLL以及从PLL进展到LSCC的敏感标志物。
在北京同仁医院收集了116例PLL和LSCC患者以及19例无发育异常患者的配对组织和血浆样本，基于石蜡包埋的肿瘤组织和血浆，采用液滴数字PCR和定量实时聚合酶链反应分析miR-21。
与对照组相比，PLL组和LSCC组组织及血浆中的miR-21水平均显著升高（两组组织与对照组相比：p < 0.0001；两组血浆与对照组相比：p < 0.05）。组织miR-21的受试者工作特征曲线下面积（AUC），PLL组与对照组相比为0.9026，LSCC组与对照组相比为0.8681。血浆miR-21的AUC，PLL组与对照组相比为0.7355，LSCC组与对照组相比为0.6723。
