Chen Shao-Jun, Yao Xu-Dong, Peng B O, Xu Yun-Fei, Wang Guang-Chun, Huang Jianhua, Liu Min, Zheng Jun-Hua
Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, P.R. China.
Exp Ther Med. 2016 Apr;11(4):1243-1248. doi: 10.3892/etm.2016.3050. Epub 2016 Feb 8.
The anticancer properties of epigallocatechin-3-gallate (EGCG) are documented in the treatment of several types of cancer; however, there is no relevant evidence for its efficacy in the treatment of renal cell carcinoma (RCC). In the present study, the therapeutic effects of EGCG were investigated, with particular attention to the metastatic behavior of human RCC cells. MTT assays and flow cytometry were performed to detect the effects of EGCG on the proliferation and apoptosis of RCC cells. The migration and invasion abilities of RCC cells following treatment with EGCG were assessed by wound-healing and Transwell assays, respectively. Gelatin zymography and western blot analysis were performed to analyze the effect of EGCG on matrix metalloproteinase-2 (MMP-2) and MMP-9 expression levels. The results suggested that EGCG was able to inhibit the proliferation of RCC cells, induce apoptosis and effectively suppressed the migration and invasion of RCC cells. In addition, EGCG treatment resulted in the downregulation of MMP-2 and MMP-9 in RCC cells. We hypothesize that the anticancer effect associated with EGCG may involve the downregulation of MMP-2 and MMP-9. The present results suggest the potential of EGCG as a novel therapeutic agent against RCC.
表没食子儿茶素-3-没食子酸酯（EGCG）的抗癌特性在多种癌症的治疗中都有记载；然而，尚无相关证据表明其对肾细胞癌（RCC）的治疗效果。在本研究中，对EGCG的治疗效果进行了研究，特别关注人肾癌细胞的转移行为。采用MTT法和流式细胞术检测EGCG对肾癌细胞增殖和凋亡的影响。分别通过伤口愈合试验和Transwell试验评估EGCG处理后肾癌细胞的迁移和侵袭能力。采用明胶酶谱法和蛋白质印迹分析来分析EGCG对基质金属蛋白酶-2（MMP-2）和MMP-9表达水平的影响。结果表明，EGCG能够抑制肾癌细胞的增殖，诱导细胞凋亡，并有效抑制肾癌细胞的迁移和侵袭。此外，EGCG处理导致肾癌细胞中MMP-2和MMP-9表达下调。我们推测，EGCG的抗癌作用可能与MMP-2和MMP-9的下调有关。目前的结果表明EGCG作为一种新型抗肾细胞癌治疗药物的潜力。
