Radwan M, Jurewicz J, Merecz-Kot D, Sobala W, Radwan P, Bochenek M, Hanke W
Department of Gynecology and Reproduction, 'Gameta' Hospital, Rzgów, Poland.
Department of Environmental Epidemiology, Nofer Institute of Occupational Medicine, Lodz, Poland.
Int J Impot Res. 2016 Jul;28(4):148-54. doi: 10.1038/ijir.2016.15. Epub 2016 Apr 14.
The clinical significance of sperm DNA damage lies in its association with natural conception rates and also might have a serious consequence on developmental outcome of the newborn. The aim of the present study is to determine whether stress and everyday life factors are associated with sperm DNA damage in adult men. The study population consisted of 286 men who attended the infertility clinic for diagnostic purposes and who had normal semen concentration of 20-300 m ml(-1) or with slight oligozoospermia (semen concentration of 15-20 m ml(-1)) (WHO, 1999). Participants were interviewed and provided a semen sample. The sperm chromatin structure assay was assessed using flow cytometry. In the present study, we found evidence for a relationship between sperm DNA damage parameters and everyday life factors. High and medium level of occupational stress and age increase DNA fragmentation index (P=0.03, P=0.004 and P=0.03, respectively). Other lifestyle factors that were positively associated with percentage of immature sperms (high DNA stainability index) included: obesity and cell phone use for more than 10 years (P=0.02 and P=0.04, respectively). Our findings indicate that stress and lifestyle factor may affect sperm DNA damage. Data from the present study showed a significant effect of age, obesity, mobile phone radiation and occupational stress on sperm DNA damage. As DNA fragmentation represents an extremely important parameter indicative of infertility and potential outcome of assisted reproduction treatment, and most of the lifestyle factors are easily modifiable, the information about factors that may affect DNA damage are important.
精子DNA损伤的临床意义在于其与自然受孕率的关联，并且可能对新生儿的发育结局产生严重影响。本研究的目的是确定压力和日常生活因素是否与成年男性的精子DNA损伤相关。研究人群包括286名因诊断目的前往不孕不育诊所就诊的男性，他们的精液浓度正常，为20 - 300 m ml(-1)，或患有轻度少精子症（精液浓度为15 - 20 m ml(-1)）（世界卫生组织，1999年）。对参与者进行了访谈并采集了精液样本。使用流式细胞术评估精子染色质结构分析。在本研究中，我们发现了精子DNA损伤参数与日常生活因素之间存在关联的证据。高水平和中等水平的职业压力以及年龄增长会增加DNA碎片化指数（分别为P = 0.03、P = 0.004和P = 0.03）。与未成熟精子百分比（高DNA染色性指数）呈正相关的其他生活方式因素包括：肥胖和使用手机超过10年（分别为P = 0.02和P = 0.04）。我们的研究结果表明，压力和生活方式因素可能会影响精子DNA损伤。本研究的数据显示年龄、肥胖、手机辐射和职业压力对精子DNA损伤有显著影响。由于DNA碎片化是表明不孕不育和辅助生殖治疗潜在结局的一个极其重要的参数，并且大多数生活方式因素易于改变，因此关于可能影响DNA损伤的因素的信息很重要。
