Division of Endocrinology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
Department of Medical Oncology, The First Affiliated Hospital, Xinxiang Medical University, Weihui, China.
Endocrinol Metab (Seoul). 2016 Jun;31(2):328-35. doi: 10.3803/EnM.2016.31.2.328. Epub 2016 Apr 8.
Resveratrol (RSV) is a polyphenolic phytoalexin that has many effects on metabolic diseases such as diabetes and obesity. Given the importance of brown adipose tissue (BAT) for energy expenditure, we investigated the effects of RSV on brown adipocytes.
For the in vitro study, interscapular BAT was isolated from 7-week-old male Sprague Dawley rats. For the in vivo study, 7-week-old male Otsuka Long Evans Tokushima Fatty (OLETF) rats were divided into four groups and treated for 27 weeks with: standard diet (SD); SD+RSV (10 mg/kg body weight, daily); high fat diet (HFD); HFD+RSV. RSV was provided via oral gavage once daily during the in vivo experiments.
RSV treatment of primary cultured brown preadipocytes promoted mitochondrial activity, along with over-expression of estrogen receptor α (ER-α). In OLETF rats, both HFD and RSV treatment increased the weight of BAT and the differentiation of BAT. However, only RSV increased the mitochondrial activity and ER-α expression of BAT in the HFD-fed group. Finally, RSV improved the insulin sensitivity of OLETF rats by increasing the mitochondrial activity of BAT, despite having no effects on white adipocytes and muscles in either diet group.
RSV could improve insulin resistance, which might be associated with mitochondrial activity of brown adipocyte. Further studies evaluating the activity of RSV for both the differentiation and mitochondrial activity of BAT could be helpful in investigating the effects of RSV on metabolic parameters.
白藜芦醇（RSV）是一种多酚类植物抗毒素，对糖尿病和肥胖等代谢性疾病有多种作用。鉴于棕色脂肪组织（BAT）对能量消耗的重要性，我们研究了 RSV 对棕色脂肪细胞的影响。
在体外研究中，从 7 周龄雄性 Sprague Dawley 大鼠分离肩胛间 BAT。在体内研究中，将 7 周龄雄性 Otsuka Long Evans Tokushima Fatty（OLETF）大鼠分为四组，用以下方法处理 27 周：标准饮食（SD）；SD+RSV（10mg/kg 体重，每日）；高脂肪饮食（HFD）；HFD+RSV。在体内实验中，通过口服灌胃每天一次给予 RSV。
RSV 处理原代培养的棕色前体脂肪细胞可促进线粒体活性，并过表达雌激素受体α（ER-α）。在 OLETF 大鼠中，HFD 和 RSV 处理均增加了 BAT 的重量和分化。然而，只有 RSV 增加了 HFD 喂养组 BAT 的线粒体活性和 ER-α表达。最后，RSV 通过增加 BAT 的线粒体活性改善了 OLETF 大鼠的胰岛素敏感性，尽管在两种饮食组中对白色脂肪细胞和肌肉均无影响。
RSV 可以改善胰岛素抵抗，这可能与棕色脂肪细胞的线粒体活性有关。进一步研究 RSV 对 BAT 的分化和线粒体活性的活性可能有助于研究 RSV 对代谢参数的影响。
