Jones Jeffrey R, Zhang Su-Chun
Waisman Center, University of Wisconsin, Madison, WI 53705, United States.
Waisman Center, University of Wisconsin, Madison, WI 53705, United States; Department of Neuroscience, University of Wisconsin, Madison, WI 53705, United States; Department of Neurology, University of Wisconsin, Madison, WI 53705, United States.
Curr Opin Biotechnol. 2016 Aug;40:133-138. doi: 10.1016/j.copbio.2016.03.010. Epub 2016 Apr 12.
The utility of human pluripotent stem cells (hPSCs) depends on their ability to produce functional cells and tissues of the body. Two strategies have been developed: directed differentiation of enriched populations of cells that match a regional and functional profile and spontaneous generation of three-dimensional organoids that resemble tissues in the body. Genomic editing of hPSCs and their differentiated cells broadens the use of the hPSC paradigm in studying human cellular function and disease as well as developing therapeutics.
人类多能干细胞（hPSC）的效用取决于它们产生身体功能性细胞和组织的能力。目前已开发出两种策略：使富集的细胞群体定向分化，使其与特定区域和功能特征相匹配；自发生成类似于体内组织的三维类器官。对hPSC及其分化细胞进行基因组编辑，拓宽了hPSC模式在研究人类细胞功能和疾病以及开发治疗方法方面的应用。
