从巴西腹泻仔猪中分离出一株不寻常的G26P[13] A组轮状病毒毒株。
Isolation of an unusual G26P[13] group A rotavirus strain from piglets with diarrhea in Brazil.
作者信息
Lorenzetti Elis, Alfieri Alice Fernandes, Alfieri Amauri Alcindo
机构信息
Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Campus Universitário, P.O. Box 10011, Londrina, Paraná, 86057-970, Brazil.
Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
出版信息
Virus Genes. 2016 Aug;52(4):586-91. doi: 10.1007/s11262-016-1336-8. Epub 2016 Apr 15.
This report describes the identification of an unusual G26P[13] genotype combination in a porcine group A rotavirus (RVA) strain. In a retrospective study, the VP7 (G type) and VP4 (P type) genes of porcine RVA Brazilian field strains identified in two diarrheic suckling piglets were amplified by RT-PCR and subjected to sequencing. The sequence analysis revealed the G26P[13] RVA genotype in one strain (BRA381) and G26P[X] in the other (BRA382). This study presents evidence of porcine RVA G26 genotype circulating in a Brazilian pig herd.
本报告描述了在一株猪A组轮状病毒（RVA）中鉴定出的一种不寻常的G26P[13]基因型组合。在一项回顾性研究中，通过逆转录聚合酶链反应（RT-PCR）扩增了在两只腹泻哺乳仔猪中鉴定出的巴西猪RVA野毒株的VP7（G型）和VP4（P型）基因，并进行了测序。序列分析显示，一株（BRA381）为G26P[13] RVA基因型，另一株（BRA382）为G26P[X]。本研究提供了猪RVA G26基因型在巴西猪群中传播的证据。
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