Deeba Farah, Pandey Ashutosh K, Pandey Vivek
Plant Ecology and Environmental Science, CSIR-National Botanical Research Institute Lucknow, India.
Front Plant Sci. 2016 Apr 8;7:425. doi: 10.3389/fpls.2016.00425. eCollection 2016.
To explore molecular mechanisms underlying the physiological response of Selaginella bryopteris, a comprehensive proteome analysis was carried out in roots and fronds undergoing dehydration and rehydration. Plants were dehydrated for 7 days followed by 2 and 24 h of rehydration. In roots out of 59 identified spots, 58 protein spots were found to be up-regulated during dehydration stress. The identified proteins were related to signaling, stress and defense, protein and nucleotide metabolism, carbohydrate and energy metabolism, storage and epigenetic control. Most of these proteins remained up-regulated on first rehydration, suggesting their role in recovery phase also. Among the 90 identified proteins in fronds, about 49% proteins were up-regulated during dehydration stress. Large number of ROS scavenging proteins was enhanced on dehydration. Many other proteins involved in energy, protein turnover and nucleotide metabolism, epigenetic control were also highly upregulated. Many photosynthesis related proteins were upregulated during stress. This would have helped plant to recover rapidly on rehydration. This study provides a comprehensive picture of different cellular responses elucidated by the proteome changes during dehydration and rehydration in roots and fronds as expected from a well-choreographed response from a resurrection plant.
为了探究卷柏生理反应背后的分子机制，对经历脱水和复水过程的根和叶进行了全面的蛋白质组分析。将植物脱水7天，随后进行2小时和24小时的复水。在根中，59个鉴定出的斑点中，有58个蛋白质斑点在脱水胁迫期间被上调。鉴定出的蛋白质与信号传导、胁迫和防御、蛋白质和核苷酸代谢、碳水化合物和能量代谢、储存及表观遗传控制有关。这些蛋白质中的大多数在首次复水时仍保持上调状态，表明它们在恢复阶段也发挥作用。在叶中鉴定出的90种蛋白质中，约49%的蛋白质在脱水胁迫期间被上调。大量的活性氧清除蛋白在脱水时增加。许多参与能量、蛋白质周转和核苷酸代谢、表观遗传控制的其他蛋白质也高度上调。许多与光合作用相关的蛋白质在胁迫期间上调。这将有助于植物在复水时迅速恢复。正如复苏植物精心编排的反应所预期的那样，这项研究提供了一幅由根和叶脱水及复水过程中蛋白质组变化所阐明的不同细胞反应的全面图景。
