Gunja-Smith Z, Lin J, Woessner J F
Department of Medicine, University of Miami School of Medicine, FL 33101.
Matrix. 1989 Jan;9(1):21-7. doi: 10.1016/s0934-8832(89)80014-9.
The wet weight of the rat uterus increased 8-fold during pregnancy and fell by 70% within 5 days postpartum. Uterine collagen increased about 5-fold during pregnancy and also fell by 70% within 5 days. The crosslink pyridinoline remained constant at 0.28 mole/mole collagen at every time point, with the possible exception of 11-12 days of pregnancy. The pyridinoline link can therefore form within the short time span of a few days, a feature presumed to be necessary to maintain the full mechanical strength of the uterus during labor. Uterine elastin increased about 8-fold during pregnancy, but the desmosines did not keep pace and fell from a normal value of 1.43 mole/mole elastin to a low of 0.89 at term. Moreover, elastin content reached a maximum several days prior to parturition and then declined continuously to 5 days postpartum. During this decline there was a selective loss of the poorly crosslinked elastin. The desmosines cannot be used as a direct measure of uterine elastin content, because of their continuously changing levels. Desmosines and pyridinoline were measured both by ELISA and by the amino acid analyzer. The two methods gave almost identical results when elastin and collagen were first separated from each other.
大鼠子宫的湿重在孕期增加了8倍，产后5天内下降了70%。子宫胶原蛋白在孕期增加了约5倍，在5天内也下降了70%。除了孕期11 - 12天可能例外，交联吡啶啉在每个时间点均保持在0.28摩尔/摩尔胶原蛋白的恒定水平。因此，吡啶啉交联可在几天的短时间内形成，这一特性被认为是分娩期间维持子宫完整机械强度所必需的。子宫弹性蛋白在孕期增加了约8倍，但锁链素并未同步增加，足月时从正常的1.43摩尔/摩尔弹性蛋白降至0.89的低值。此外，弹性蛋白含量在分娩前几天达到最大值，然后持续下降至产后5天。在这一下降过程中，交联较差的弹性蛋白出现选择性丢失。由于锁链素水平不断变化，因此不能将其用作子宫弹性蛋白含量的直接测量指标。锁链素和吡啶啉均通过酶联免疫吸附测定法（ELISA）和氨基酸分析仪进行测量。当弹性蛋白和胶原蛋白首先彼此分离时，两种方法给出的结果几乎相同。
