Fortunato Tiago M, Beltrami Cristina, Emanueli Costanza, De Bank Paul A, Pula Giordano
Department of Pharmacy and Pharmacology, University of Bath, Bath, UK.
Bristol Heart Institute, School of Clinical Sciences University of Bristol, Bristol, UK.
Sci Rep. 2016 May 4;6:25326. doi: 10.1038/srep25326.
Revascularisation is a key step for tissue regeneration and complete organ engineering. We describe the generation of human platelet lysate gel (hPLG), an extracellular matrix preparation from human platelets able to support the proliferation of endothelial colony forming cells (ECFCs) in 2D cultures and the formation of a complete microvascular network in vitro in 3D cultures. Existing extracellular matrix preparations require addition of high concentrations of recombinant growth factors and allow only limited formation of capillary-like structures. Additional advantages of our approach over existing extracellular matrices are the absence of any animal product in the composition hPLG and the possibility of obtaining hPLG from patients to generate homologous scaffolds for re-implantation. This discovery has the potential to accelerate the development of regenerative medicine applications based on implantation of microvascular networks expanded ex vivo or the generation of fully vascularised organs.
血管再生是组织再生和完整器官工程的关键步骤。我们描述了人血小板裂解物凝胶（hPLG）的生成，这是一种从人血小板制备的细胞外基质，能够支持内皮集落形成细胞（ECFCs）在二维培养中的增殖，并在三维培养中体外形成完整的微血管网络。现有的细胞外基质制剂需要添加高浓度的重组生长因子，并且仅允许有限地形成毛细血管样结构。我们的方法相对于现有细胞外基质的其他优点包括hPLG成分中不存在任何动物产品，以及有可能从患者身上获得hPLG以生成用于再植入的同源支架。这一发现有可能加速基于体外扩增的微血管网络植入或完全血管化器官生成的再生医学应用的发展。
