Kumar Purnima S, Leys Eugene J, Bryk Jennifer M, Martinez Francisco J, Moeschberger Melvin L, Griffen Ann L
The Ohio State University, Section of Pediatric Dentistry, 305 W. 12th Ave., P.O. Box 182357, Columbus, OH 43218, USA.
J Clin Microbiol. 2006 Oct;44(10):3665-73. doi: 10.1128/JCM.00317-06.
The gingival sulcus contains a complex ecosystem that includes many uncultivated bacteria. Understanding the dynamics of this ecosystem in transitions between health and disease is important in advancing our understanding of the bacterial etiology of periodontitis. The objective of this longitudinal study was to examine the stability of bacterial colonization in the gingival crevice and to explore the relationship between shifts in microbial composition and changes in periodontal health status using a comprehensive, quantitative, culture-independent approach. Subgingival plaque samples and periodontal data were collected from 24 subjects over 2 years. Baseline and 2-year plaque samples were analyzed using quantitative ribosomal 16S cloning and sequencing. Ten subjects remained periodontally healthy over 2 years, the periodontal health of seven subjects worsened, and seven subjects showed clinical improvement. Bacterial stability was greatest among healthy, clinically stable subjects and lowest for subjects whose periodontal status worsened (P = 0.01). Higher numbers of species lost or gained were also observed for subjects whose clinical status changed (P = 0.009). This provides evidence that a change in periodontal status is accompanied by shifts within the bacterial community. Based on these data, measures of microbial stability may be useful in clinical diagnosis and prognosis. Regarding individual species, increases in levels of the uncultivated phylotype Veillonella sp. oral clone X042, a gram-negative bacterium and the most common member of the subgingival bacterial community, were associated with periodontal health (P = 0.04), suggesting that this is an important beneficial species. Filifactor alocis, a gram-positive anaerobe, was found at higher levels in subjects with disease (P = 0.01).
牙龈沟包含一个复杂的生态系统，其中包括许多未培养的细菌。了解该生态系统在健康与疾病转变过程中的动态变化，对于深化我们对牙周炎细菌病因的理解至关重要。这项纵向研究的目的是，使用一种全面、定量、不依赖培养的方法，检查牙龈裂隙中细菌定植的稳定性，并探索微生物组成变化与牙周健康状况变化之间的关系。在两年时间里，从24名受试者身上收集了龈下菌斑样本和牙周数据。使用定量核糖体16S克隆和测序技术分析了基线和两年时的菌斑样本。24名受试者中，10名在两年间保持牙周健康，7名受试者的牙周健康状况恶化，7名受试者的临床状况有所改善。细菌稳定性在健康、临床稳定的受试者中最高，在牙周状况恶化的受试者中最低（P = 0.01）。临床状况发生变化的受试者，其失去或获得的物种数量也更多（P = 0.009）。这为牙周状况的变化伴随着细菌群落内部的转变提供了证据。基于这些数据，微生物稳定性的测量指标可能对临床诊断和预后有用。就个别物种而言，未培养的韦荣氏菌属种口腔克隆X042（一种革兰氏阴性菌，也是龈下细菌群落中最常见的成员）水平的增加与牙周健康相关（P = 0.04），这表明它是一种重要的有益物种。在患病受试者中发现，革兰氏阳性厌氧菌嗜栖丝杆菌的水平较高（P = 0.01）。
