a Infectious Diseases and Clinical Microbiology Department , Yildirim Beyazit University, Ankara Ataturk Training & Research Hospital , Ankara , Turkey.
b Infectious Diseases and Clinical Microbiology Department , Adiyaman University, Faculty of Medicine , Adiyaman , Turkey.
Redox Rep. 2017 Nov;22(6):241-245. doi: 10.1080/13510002.2016.1178481. Epub 2016 May 9.
Crimean Congo hemorrhagic fever (CCHF) is the second most common hemorrhagic fever worldwide. This study aimed to evaluate the oxidant-antioxidant balance of patients with CCHF by detecting dynamic thiol disulfide homeostasis (TDH), which is a novel oxidative stress marker, and other molecules, including paraoxonase (PON), arylesterase (ARES), ceruloplasmin (CLP), myeloperoxidase (MPO), and catalase.
This retrospective, cross-sectional, controlled study, which involved patients with CCHF and healthy volunteers, measured dynamic TDH using a novel automated method developed by Erel.
We recruited 69 adult patients with CCHF (31 females, 38 males, median age 46 years). The case fatality rate was 1.49% (1/69). Increased disulfide/native thiol and disulfide/total thiol ratios, decreased total antioxidant status (TAS), and increased total oxidant status (TOS) were found in patients with CCHF. TAS, PON, and ARES values were found to be positively correlated with both native and total thiol levels, whereas TOS and CLP were negatively correlated with both, at a significant level. MPO activity was similar in both groups.
This is the first study in the literature to evaluate dynamic TDH in CCHF. TDH shifts to the oxidative side in patients with CCHF, leading to an increase in TOS.
克里米亚-刚果出血热（CCHF）是全球第二常见的出血热。本研究旨在通过检测新型氧化应激标志物——动态硫醇二硫化物平衡（TDH）以及其他分子（包括对氧磷酶（PON）、芳基酯酶（ARES）、铜蓝蛋白（CLP）、髓过氧化物酶（MPO）和过氧化氢酶）来评估 CCHF 患者的氧化还原平衡。
这是一项回顾性、横断面、对照研究，纳入了 CCHF 患者和健康志愿者，使用 Erel 开发的新型自动化方法来测量动态 TDH。
我们共招募了 69 例成年 CCHF 患者（31 名女性，38 名男性，中位年龄 46 岁）。病死率为 1.49%（1/69）。CCHF 患者的二硫化物/天然硫醇和二硫化物/总硫醇比值升高，总抗氧化状态（TAS）降低，总氧化状态（TOS）升高。TAS、PON 和 ARES 值与天然和总硫醇水平呈正相关，而 TOS 和 CLP 与两者均呈负相关，差异有统计学意义。两组 MPO 活性相似。
这是文献中首次评估 CCHF 中的动态 TDH。CCHF 患者的 TDH 向氧化侧偏移，导致 TOS 增加。
