Center for RNA Nanobiotechnology and Nanomedicine, College of Pharmacy, Dorothy M. Davis Heart and Lung Research Institute, James Comprehensive Cancer Center, College of Medicine, The Ohio State University, Columbus, OH 43210, USA.
OSU Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.
Nanomedicine. 2023 Feb;48:102642. doi: 10.1016/j.nano.2022.102642. Epub 2022 Dec 26.
Detection of cancer in its early stage is a challenging task for oncologists. Inflammatory breast cancer has symptoms that are similar to mastitis and can be mistaken for microbial infection. Currently, the differential diagnosis between mastitis and Inflammatory breast cancer via nipple aspirate fluid (NAF) is difficult. Here, we report a label-free and amplification-free detection platform using an engineered nanopore of the phi29 DNA-packaging motor with biomarker Galectin3 (GAL3), Thomsen-Friedenreich (TF) binding peptide as the probe fused at its C-terminus. The binding of the biomarker in NAF samples from breast cancer patients to the probe results in the connector's conformational change with a current blockage of 32 %. Utilization of dwell time, blockage ratio, and peak signature enable us to detect basal levels of biomarkers from patient NAF samples at the single-molecule level. This platform will allow for breast cancers to be resolved at an early stage with accuracy and thoroughness.
早期癌症的检测对于肿瘤学家来说是一项具有挑战性的任务。炎性乳腺癌的症状类似于乳腺炎，容易被误诊为微生物感染。目前，通过乳头吸出液（NAF）鉴别乳腺炎和炎性乳腺癌比较困难。在这里，我们报告了一种无标记和无扩增的检测平台，该平台使用了一种经过工程改造的 phi29 DNA 包装马达的纳米孔，其 C 末端融合了生物标志物半乳糖凝集素 3（GAL3）和 Thomssen-Friedenreich（TF）结合肽作为探针。在来自乳腺癌患者的 NAF 样本中，生物标志物与探针的结合导致连接器的构象发生变化，电流阻断率为 32%。利用停留时间、阻断率和峰特征，我们能够在单细胞水平上检测到来自患者 NAF 样本的生物标志物的基础水平。该平台将能够早期准确全面地诊断乳腺癌。
