Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
Mikrochim Acta. 2018 Dec 10;186(1):11. doi: 10.1007/s00604-018-3129-3.
A surface-enhanced Raman scattering (SERS) based nanoprobe was developed for detection and imaging of endogenous peroxynitrite in living cells. The probe was fabricated by assembling 3-mercaptophenylboronic acid pinacol ester onto the surface of gold nanoparticles (AuNPs). The detection of peroxynitrite is accomplished via measurement of the changes in the SERS spectra (at 882 cm) that are caused by the reaction between probe and peroxynitrite. The probe has a fast response (<30 s), a 0.4 μM lower detection limit and a wide linearity range from 5.0 × 10 to 1.0 × 10 M. It is biocompatible and highly stable on storage and under various pH conditions. Both the reaction and the SERS signal are highly specific over other species. The nanoprobe was successfully applied to SERS imaging of peroxynitrite that is produced in macrophages under oxidative stress. Conceivably, the method has a most viable tool for use in studies on peroxynitrite-related physiological and pathological processes. Graphical abstract Schematic presentation of surface-enhanced Raman scattering (SERS) nanoprobes fabricated by assembling phenylboronate on gold nanoparticles (AuNPs) for detecting intracellular peroxynitrite (ONOO) via specific reaction-caused SERS changes.
一种基于表面增强拉曼散射（SERS）的纳米探针被开发用于检测和成像活细胞内源性过氧亚硝酸盐。该探针通过将 3-巯基苯硼酸频哪醇酯组装到金纳米粒子（AuNPs）表面来制备。通过测量探针与过氧亚硝酸盐反应引起的 SERS 光谱（在 882cm-1 处）的变化来检测过氧亚硝酸盐。该探针具有快速响应（<30s）、0.4μM 的低检测限和 5.0×10-6至 1.0×10-5M 的宽线性范围。它在储存和各种 pH 条件下具有良好的生物相容性和高度稳定性。与其他物质相比，反应和 SERS 信号都具有高度特异性。该纳米探针成功地应用于在氧化应激下巨噬细胞中产生的过氧亚硝酸盐的 SERS 成像。可以想象，该方法为研究过氧亚硝酸盐相关的生理和病理过程提供了一种最可行的工具。示意图 展示了通过将苯硼酸酯组装到金纳米粒子（AuNPs）上来制备表面增强拉曼散射（SERS）纳米探针，用于通过特定反应引起的 SERS 变化来检测细胞内过氧亚硝酸盐（ONOO-）。
