Cinpolat Ovgu, Unal Zeynep Nil, Ismi Onur, Gorur Aysegul, Unal Murat
Gaziantep State Hospital, Gaziantep, Turkey.
University of Mersin, Faculty of Pharmacy, Department of Biochemistry, Mersin, Turkey.
Braz J Otorhinolaryngol. 2017 May-Jun;83(3):276-284. doi: 10.1016/j.bjorl.2016.03.013. Epub 2016 Apr 27.
Salivary gland tumors (SGTs) are rare head and neck malignancies consisting of a spectrum of tumors with different biological behaviors.
In this study we aimed to find out differential expression of microRNA profiles between benign and malignant SGTs.
We investigated the possible role of 95 microRNAs in the 20 patients with salivary gland tumors with comparison of 17 patients without malignancy or salivary gland diseases. Sixteen of the tumors were benign (seven pleomorphic adenomas, nine Warthin tumors), four of them were malignant (two squamous cell carcinomas, one high grade mucoepidermoid carcinoma, one adenocarcinoma). Serum and saliva samples were collected from both patients and control group. Tissue samples of tumor masses were also collected from patient group.
Among studied microRNAs miR-21, miR-23a, miR-27a, miR-223, miR-125b, miR-126, miR-146a, miR-30e were down regulated in the benign group compared to control group in the serum samples (p-values are 0.04, 0.00005, 0.00005, 0.0022, 0.031, 0.00008, 0.044, and 0.0007, respectively). When tissue samples were studied miR-21, miR-31, miR-199a-5p, miR-146b, miR-345 were up-regulated in the malignant group compared to benign group (p values are 0.006, 0.02, 0.013, 0.013, 0.041, respectively). miR-30e showed statistically significant up-regulation in malignant tumor group's plasma samples compared to benign group (p=0.034). There was no statistically significant difference in saliva samples between groups.
Our results showed that different microRNAs may play role in salivary tumor pathogenesis according to biological behavior. Although there was no difference in saliva samples between groups, according to tissue and serum samples miR-21 and 30e may have an important role; since they were down-regulated in benign tumors whereas up-regulated in malignant ones.
涎腺肿瘤（SGTs）是罕见的头颈部恶性肿瘤，由一系列具有不同生物学行为的肿瘤组成。
在本研究中，我们旨在找出良性和恶性涎腺肿瘤之间微小RNA谱的差异表达。
我们调查了95种微小RNA在20例涎腺肿瘤患者中的可能作用，并与17例无恶性肿瘤或涎腺疾病的患者进行比较。其中16例肿瘤为良性（7例多形性腺瘤，9例沃辛瘤），4例为恶性（2例鳞状细胞癌，1例高级别黏液表皮样癌，1例腺癌）。从患者组和对照组收集血清和唾液样本。还从患者组收集肿瘤块的组织样本。
在所研究的微小RNA中，血清样本中，与对照组相比，良性组中的miR-21、miR-23a、miR-27a、miR-223、miR-125b、miR-126、miR-146a、miR-30e表达下调（p值分别为0.04、0.00005、0.00005、0.0022、0.031、0.00008、0.044和0.0007）。在研究组织样本时，与良性组相比，恶性组中的miR-21、miR-31、miR-199a-5p、miR-146b、miR-345表达上调（p值分别为0.006、0.02、0.013、0.013、0.041）。与良性组相比，恶性肿瘤组血浆样本中的miR-30e显示出统计学上的显著上调（p = 0.034）。各组唾液样本之间无统计学显著差异。
我们的结果表明，不同的微小RNA可能根据生物学行为在涎腺肿瘤发病机制中发挥作用。尽管各组唾液样本之间没有差异，但根据组织和血清样本，miR-21和30e可能起重要作用；因为它们在良性肿瘤中下调，而在恶性肿瘤中上调。
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