Yi Tao, Qi Li, Li Ji, Le Jing-Jing, Shao Lei, Du Xin, Dong Jing-Cheng
Department of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, China; College of Integrated Traditional Chinese and Western Medicine, Fudan University, Shanghai, China.
E-institute of Shanghai Municipal Education Committee, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Neural Regen Res. 2016 Apr;11(4):610-6. doi: 10.4103/1673-5374.180746.
In China, moxibustion is reported to be useful and has few side effects for chronic fatigue syndrome, but its mechanisms are largely unknown. More recently, the focus has been on the wealth of information supporting stress as a factor in chronic fatigue syndrome, and largely concerns dysregulation in the stress-related hypothalamic-pituitary-adrenal axis. In the present study, we aimed to determine the effect of moxibustion on behavioral symptoms in chronic fatigue syndrome rats and examine possible mechanisms. Rats were subjected to a combination of chronic restraint stress and forced swimming to induce chronic fatigue syndrome. The acupoints Guanyuan (CV4) and Zusanli (ST36, bilateral) were simultaneously administered moxibustion. Untreated chronic fatigue syndrome rats and normal rats were used as controls. Results from the forced swimming test, open field test, tail suspension test, real-time PCR, enzyme-linked immunosorbent assay, and western blot assay showed that moxibustion treatment decreased mRNA expression of corticotropin-releasing hormone in the hypothalamus, and adrenocorticotropic hormone and corticosterone levels in plasma, and markedly increased progranulin mRNA and protein expression in the hippocampus. These findings suggest that moxibustion may relieve the behavioral symptoms of chronic fatigue syndrome, at least in part, by modulating the hypothalamic-pituitary-adrenal axis and upregulating hippocampal progranulin.
在中国，据报道艾灸对慢性疲劳综合征有效且副作用少，但其机制大多未知。最近，重点已放在大量支持压力作为慢性疲劳综合征一个因素的信息上，且很大程度上涉及与压力相关的下丘脑 - 垂体 - 肾上腺轴的失调。在本研究中，我们旨在确定艾灸对慢性疲劳综合征大鼠行为症状的影响并研究可能的机制。大鼠接受慢性束缚应激和强迫游泳相结合的处理以诱导慢性疲劳综合征。对关元穴（CV4）和足三里穴（双侧ST36）同时进行艾灸。未处理的慢性疲劳综合征大鼠和正常大鼠用作对照。强迫游泳试验、旷场试验、尾悬挂试验、实时聚合酶链反应、酶联免疫吸附测定和蛋白质印迹分析的结果表明，艾灸处理降低了下丘脑促肾上腺皮质激素释放激素的mRNA表达以及血浆中促肾上腺皮质激素和皮质酮水平，并显著增加了海马中颗粒体蛋白前体的mRNA和蛋白质表达。这些发现表明，艾灸可能至少部分地通过调节下丘脑 - 垂体 - 肾上腺轴和上调海马颗粒体蛋白前体来缓解慢性疲劳综合征的行为症状。
