Williams K P, Müller R, Rüger W, Geiduschek E P
Department of Biology, University of California, San Diego, La Jolla 92093.
J Bacteriol. 1989 Jun;171(6):3579-82. doi: 10.1128/jb.171.6.3579-3582.1989.
Bacteriophage T4 gene 33 protein (gp33), which is required for viral late transcription, has been overproduced. The purified gp33 binds to RNA polymerase core from uninfected or T4-infected Escherichia coli, but the major E. coli transcription initiation factor, sigma 70, competed effectively for this binding.
噬菌体T4基因33蛋白(gp33)是病毒晚期转录所必需的,现已大量产生。纯化后的gp33可与未感染或T4感染的大肠杆菌的RNA聚合酶核心结合,但主要的大肠杆菌转录起始因子σ70能有效地竞争这种结合。