Mi Hongfei, Wang Dai, Xue Yunxin, Zhang Zhi, Niu Jianjun, Hong Yuzhi, Drlica Karl, Zhao Xilin
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiang-An District, Xiamen, Fujian Province, China School of Public Health, Fujian Medical University, Minhou District, Fuzhou, Fujian Province, China.
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiang-An District, Xiamen, Fujian Province, China.
Antimicrob Agents Chemother. 2016 Jul 22;60(8):5054-8. doi: 10.1128/AAC.03003-15. Print 2016 Aug.
The contribution of reactive oxygen species (ROS) to antimicrobial lethality was examined by treating Escherichia coli with dimethyl sulfoxide (DMSO), an antioxidant solvent frequently used in antimicrobial studies. DMSO inhibited killing by ampicillin, kanamycin, and two quinolones and had little effect on MICs. DMSO-mediated protection correlated with decreased ROS accumulation and provided evidence for ROS-mediated programmed cell death. These data support the contribution of ROS to antimicrobial lethality and suggest caution when using DMSO-dissolved antimicrobials for short-time killing assays.
通过用二甲基亚砜（DMSO）处理大肠杆菌来检测活性氧（ROS）对抗菌杀伤力的作用，DMSO是抗菌研究中常用的一种抗氧化剂溶剂。DMSO抑制氨苄青霉素、卡那霉素和两种喹诺酮类药物的杀菌作用，对最低抑菌浓度（MIC）影响很小。DMSO介导的保护作用与ROS积累减少相关，并为ROS介导的程序性细胞死亡提供了证据。这些数据支持ROS对抗菌杀伤力的作用，并建议在使用DMSO溶解的抗菌药物进行短期杀伤试验时要谨慎。
