Wüst Pia K, Foesel Bärbel U, Geppert Alicia, Huber Katharina J, Luckner Manja, Wanner Gerhard, Overmann Jörg
Department of Microbial Ecology and Diversity Research, Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
Department of Biology I, Ludwig-Maximilians-Universität München, Martinsried, Germany.
Int J Syst Evol Microbiol. 2016 Sep;66(9):3355-3366. doi: 10.1099/ijsem.0.001199. Epub 2016 Jun 2.
Three novel strains of the phylum Acidobacteria (Ac_11_E3T, Ac_12_G8T and Ac_16_C4T) were isolated from Namibian semiarid savanna soils by a high-throughput cultivation approach using low-nutrient growth media. 16S rRNA gene sequence analysis placed all three strains in the order Blastocatellales of the class Blastocatellia (Acidobacteria subdivision 4). However, 16S rRNA gene sequence similarities to their closest relative Pyrinomonas methylaliphatogenes K22T were ≤90 %. Cells of strains Ac_11_E3T, Ac_12_G8T and Ac_16_C4T were Gram-staining-negative and non-motile and divided by binary fission. Ac_11_E3T and Ac_16_C4T formed white colonies, while those of Ac_12_G8T were orange-yellowish. All three strains were aerobic chemoorganoheterotrophic mesophiles with a broad pH range for growth. All strains used a very limited spectrum of carbon and energy sources for growth, with a preference for complex proteinaceous substrates. The major respiratory quinone was MK-8. The major shared fatty acid was iso-C15 : 0. The DNA G+C contents of strains Ac_11_E3T, Ac_12_G8T and Ac_16_C4T were 55.9 mol%, 66.9 mol% and 54.7 mol%, respectively. Based on these characteristics, the two novel genera Brevitaleagen. nov. and Arenimicrobiumgen. nov. are proposed, harboring the novel species Brevitaleaaridisoli sp. nov. (Ac_11_E3T=DSM 27934T=LMG 28618T), Brevitalea deliciosa sp. nov. (Ac_16_C4T=DSM 29892T=LMG 28995T) and Arenimicrobium luteum sp. nov. (Ac_12_G8T=DSM 26556T=LMG 29166T), respectively. Since these novel genera are only distantly related to established families, we propose the novel family Pyrinomonadaceaefam. nov. that accommodates the proposed genera and the genus Pyrinomonas(Crowe et al., 2014).
通过使用低营养生长培养基的高通量培养方法，从纳米比亚半干旱稀树草原土壤中分离出了三株新型酸杆菌门菌株（Ac_11_E3T、Ac_12_G8T和Ac_16_C4T）。16S rRNA基因序列分析将这三株菌株都归入了芽生球菌纲芽生球菌目的酸杆菌亚群4。然而，它们与最接近的亲缘种甲基脂肪芽孢单胞菌K22T的16S rRNA基因序列相似性≤90%。Ac_11_E3T、Ac_12_G8T和Ac_16_C4T菌株的细胞革兰氏染色阴性、不运动，通过二分裂进行繁殖。Ac_11_E3T和Ac_16_C4T形成白色菌落，而Ac_12_G8T的菌落为橙黄色。这三株菌株均为需氧化学有机异养中温菌，生长pH范围较宽。所有菌株生长所用的碳源和能源种类非常有限，偏好复杂的蛋白质类底物。主要呼吸醌为MK-8。主要的共有脂肪酸为异-C15 : 0。Ac_11_E3T、Ac_12_G8T和Ac_16_C4T菌株的DNA G+C含量分别为55.9 mol% %、66.9 mol%和54.7 mol%。基于这些特征，提出了两个新属，即短柄菌属（Brevitaleagen. nov.）和沙栖微菌属（Arenimicrobiumgen. nov.），分别包含新种干旱短柄菌（Brevitaleaaridisoli sp. nov.）（Ac_11_E3T=DSM 27934T=LMG 28618T）、美味短柄菌（Brevitalea deliciosa sp. nov.）（Ac_16_C4T=DSM 29892T=LMG 28995T）和黄色沙栖微菌（Arenimicrobium luteum sp. nov.）（Ac_12_G8T=DSM 26556T=LMG 29166T）。由于这些新属与已确立的科亲缘关系较远，我们提出了新科芽孢单胞菌科（Pyrinomonadaceaefam. nov.），该科包含所提出的属以及芽孢单胞菌属（Crowe等人，2014年）。
