Arscott P G, Lee G, Bloomfield V A, Evans D F
Department of Biochemistry, University of Minnesota, St. Paul 55108.
Nature. 1989 Jun 8;339(6224):484-6. doi: 10.1038/339484a0.
Scanning tunnelling microscopy (STM) has been used to map the surface topography of inorganic materials at the atomic level, and is potentially one of the most powerful techniques for probing biomolecular structure. Recent STM studies of calf thymus DNA and poly(rA).poly(rU) have shown that the helical pitch and periodic alternation of major and minor grooves can be visualized and reliably measured. Here we present the first STM images of poly(dG-me5dC).poly(dG-me5dC) in the Z-form. Both the general appearance of the fibres and measurements of helical parameters are in good agreement with models derived from X-ray diffraction.
扫描隧道显微镜（STM）已被用于在原子水平上绘制无机材料的表面形貌，并且可能是探测生物分子结构最强大的技术之一。最近对小牛胸腺DNA和聚（rA）·聚（rU）的STM研究表明，螺旋间距以及大沟和小沟的周期性交替可以被可视化并可靠地测量。在此，我们展示了聚（dG - me5dC）·聚（dG - me5dC）Z型结构的首批STM图像。纤维的总体外观和螺旋参数测量结果与从X射线衍射得出的模型高度吻合。
