酸浆中的睡茄内酯及其对一氧化氮生成的抑制作用。
Withanolides from Physalis minima and their inhibitory effects on nitric oxide production.
作者信息
Guan Yu-Zhou, Shan Si-Ming, Zhang Wei, Luo Jian-Guang, Kong Ling-Yi
机构信息
State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
出版信息
Steroids. 2014 Apr;82:38-43. doi: 10.1016/j.steroids.2014.01.004. Epub 2014 Jan 27.
Six new withanolides (1-6), including two uncommon 1,10-seco withanolides (1 and 2), together with five known withanolides (7-11), were isolated from the whole plants of Physalis minima Linn.. The structures of new compounds were elucidated through spectroscopic methods, including (1)H, (13)C NMR, 2D-NMR, HRESIMS and circular dichroism (CD). Inhibitory effects of the isolates on nitric oxide (NO) production in lipopolysaccaride-activated RAW264.7 macrophages were evaluated. Compounds 2 and 5 showed strong inhibitory activities with IC50 values of 8.04 and 10.01 μM, respectively. Compounds 1, 9 and 10 exhibited moderate inhibitory activities with IC50 values from 25.54 to 43.58 μM.
从酸浆全草中分离出6个新的睡茄内酯（1 - 6），包括2个罕见的1,10 - 裂环睡茄内酯（1和2），以及5个已知的睡茄内酯（7 - 11）。通过光谱方法对新化合物的结构进行了鉴定，包括氢谱（¹H）、碳谱（¹³C NMR）、二维核磁共振谱（2D - NMR）、高分辨电喷雾电离质谱（HRESIMS）和圆二色光谱（CD）。评估了这些分离物对脂多糖激活的RAW264.7巨噬细胞中一氧化氮（NO）产生的抑制作用。化合物2和5表现出较强的抑制活性，IC50值分别为8.04和10.01 μM。化合物1、9和10表现出中等抑制活性，IC50值在25.54至43.58 μM之间。
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