Lin T Jennifer, El Sebae Gabriel, Jung Jong-Hyun, Jung Dong-Hyun, Park Cheon-Seok, Holden James F
Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA.
Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea.
Int J Syst Evol Microbiol. 2016 Sep;66(9):3372-3376. doi: 10.1099/ijsem.0.001201. Epub 2016 Jun 3.
A hyperthermophilic, autotrophic iron and nitrate reducer, strain Su06T, was isolated from an active deep-sea hydrothermal vent chimney on the Endeavour Segment in the north-eastern Pacific Ocean. It was obligately anaerobic, hydrogenotrophic and reduced Fe(III) oxide to magnetite and NO3- to N2. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain was more than 97 % similar to other species of the genera Pyrodictium and Hyperthermus. Therefore, overall genome relatedness index analyses were performed to establish whether strain Su06T represents a novel species. For each analysis, strain Su06T was most similar to Pyrodictium occultum PL-19T. Relative to this strain, the average nucleotide identity score for strain Su06T was 72 %, the genome-to-genome direct comparison score was 13-19 % and the species identification score at the protein level was 89 %. For each analysis, strain Su06T was below the species delineation cutoff. Based on its whole genome sequence and its unique phenotypic characteristics, strain Su06T is suggested to represent a novel species of the genus Pyrodictium, for which the name Pyrodictium delaneyi is proposed. The type strain is Su06T (=DSM 28599T=ATCC BAA-2559T).
从东北太平洋奋进段一个活跃的深海热液喷口烟囱中分离出了一株嗜热自养铁和硝酸盐还原菌，菌株Su06T。它是专性厌氧、氢营养型的，能将Fe(III)氧化物还原为磁铁矿，将NO3-还原为N2。基于16S rRNA基因序列的系统发育分析表明，该菌株与火网菌属和嗜热栖热菌属的其他物种相似度超过97%。因此，进行了全基因组相关性指数分析，以确定菌株Su06T是否代表一个新物种。在每次分析中，菌株Su06T与隐匿火网菌PL-19T最为相似。相对于该菌株，菌株Su06T的平均核苷酸同一性分数为72%，基因组到基因组直接比较分数为13%-19%，蛋白质水平的物种鉴定分数为89%。在每次分析中，菌株Su06T均低于物种界定阈值。基于其全基因组序列和独特的表型特征，菌株Su06T被认为代表火网菌属的一个新物种，为此提出名为Pyrodictium delaneyi的名称。模式菌株为Su06T(=DSM 28599T=ATCC BAA-2559T)。
