Tiemeier D C, Tilghman S M, Polsky F I, Seidman J G, Leder A, Edgell M H, Leder P
Cell. 1978 Jun;14(2):237-45. doi: 10.1016/0092-8674(78)90110-1.
The BALC/c mouse has two nonallelic beta-globin genes that appear to reside on two different Eco R1 fragments of genomic DNA. We have already cloned one of these fragments and shown that the gene encoded within it is interrupted by at least one large intervening sequence of DNA. We have now cloned and characterized the second beta-globin gene-containing fragment. The coding sequence of its gene is also interrupted by an intervening sequence of DNA that occurs in about the same position, relative to the coding sequence, as does the first. Because some shared features of the structure of these two genes might be responsible for their coordinate expression and the elimination of their intervening sequences, we have compared their surrounding, coding and intervening sequences by restriction endonuclease analysis and by visualization of the heteroduplex structures formed between them. Of the 7000 bp of sequence compared in this way, we find only a few hundred base pairs of homology in addition to the coding sequence. These shared sequences flank the coding sequence and appear to include only those portions of the intervening sequence immediately adjacent to the interrupted structural gene.
BALC/c小鼠有两个非等位的β-珠蛋白基因，它们似乎位于基因组DNA的两个不同的Eco R1片段上。我们已经克隆了其中一个片段，并表明其中编码的基因被至少一个大的DNA间隔序列打断。我们现在已经克隆并鉴定了第二个含β-珠蛋白基因的片段。其基因的编码序列也被一个DNA间隔序列打断，该间隔序列相对于编码序列而言，出现在与第一个大致相同的位置。由于这两个基因结构的一些共同特征可能是它们协调表达以及去除其间隔序列的原因，我们通过限制性内切酶分析以及观察它们之间形成的异源双链结构，比较了它们的侧翼、编码和间隔序列。通过这种方式比较的7000个碱基对序列中，除了编码序列外，我们仅发现几百个碱基对的同源性。这些共享序列位于编码序列两侧，并且似乎仅包括间隔序列中紧邻被打断的结构基因的那些部分。
