利用带有自动数据处理和分配功能的微阵列系统快速检测结核分枝杆菌复合群细菌。
Rapid spoligotyping of Mycobacterium tuberculosis complex bacteria by use of a microarray system with automatic data processing and assignment.
机构信息
Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Molecular Pathogenesis, Jena, Germany.
出版信息
J Clin Microbiol. 2012 Jul;50(7):2492-5. doi: 10.1128/JCM.00442-12. Epub 2012 May 2.
Abstract
Membrane-based spoligotyping has been converted to DNA microarray format to qualify it for high-throughput testing. We have shown the assay's validity and suitability for direct typing from tissue and detecting new spoligotypes. Advantages of the microarray methodology include rapidity, ease of operation, automatic data processing, and affordability.
摘要
膜基 spoligotyping 已被转化为 DNA 微阵列格式，以使其能够进行高通量测试。我们已经证明了该检测方法的有效性和直接从组织中进行直接分型以及检测新 spoligotype 的适用性。微阵列方法学的优点包括快速、易于操作、自动数据处理和价格合理。
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