Hong Hyobong, Lim Eul-Gyoon, Jeong Jae-Chan, Chang Jiho, Shin Sung-Woong, Krause Hans-Joachim
Advanced Vision System Research Section, Electronics & Telecommunication Research Institute (ETRI);
Intelligent Cognitive Technology Research Department, Electronics & Telecommunication Research Institute (ETRI).
J Vis Exp. 2016 Jun 9(112):53869. doi: 10.3791/53869.
The setup of a planar Frequency Mixing Magnetic Detection (p-FMMD) scanner for performing Magnetic Particles Imaging (MPI) of flat samples is presented. It consists of two magnetic measurement heads on both sides of the sample mounted on the legs of a u-shaped support. The sample is locally exposed to a magnetic excitation field consisting of two distinct frequencies, a stronger component at about 77 kHz and a weaker field at 61 Hz. The nonlinear magnetization characteristics of superparamagnetic particles give rise to the generation of intermodulation products. A selected sum-frequency component of the high and low frequency magnetic field incident on the magnetically nonlinear particles is recorded by a demodulation electronics. In contrast to a conventional MPI scanner, p-FMMD does not require the application of a strong magnetic field to the whole sample because mixing of the two frequencies occurs locally. Thus, the lateral dimensions of the sample are just limited by the scanning range and the supports. However, the sample height determines the spatial resolution. In the current setup it is limited to 2 mm. As examples, we present two 20 mm × 25 mm p-FMMD images acquired from samples with 1 µm diameter maghemite particles in silanol matrix and with 50 nm magnetite particles in aminosilane matrix. The results show that the novel MPI scanner can be applied for analysis of thin biological samples and for medical diagnostic purposes.
介绍了一种用于对扁平样品进行磁粒子成像(MPI)的平面频率混合磁检测(p-FMMD)扫描仪的设置。它由安装在U形支架腿上的位于样品两侧的两个磁测量头组成。样品局部暴露于由两个不同频率组成的磁激发场中,一个较强的分量约为77 kHz,一个较弱的场为61 Hz。超顺磁性颗粒的非线性磁化特性导致互调产物的产生。通过解调电子设备记录入射到磁性非线性颗粒上的高频和低频磁场的选定和频分量。与传统的MPI扫描仪相比,p-FMMD不需要对整个样品施加强磁场,因为两个频率的混合在局部发生。因此,样品的横向尺寸仅受扫描范围和支架的限制。然而,样品高度决定了空间分辨率。在当前设置中,它限制为2 mm。作为示例,我们展示了从硅烷醇基质中含有1 µm直径磁赤铁矿颗粒和氨基硅烷基质中含有50 nm磁铁矿颗粒的样品获得的两张20 mm×25 mm的p-FMMD图像。结果表明,这种新型MPI扫描仪可用于分析薄生物样品和用于医学诊断目的。
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