Power Krista A, Lu Jenifer T, Monk Jennifer M, Lepp Dion, Wu Wenqing, Zhang Claire, Liu Ronghua, Tsao Rong, Robinson Lindsay E, Wood Geoffrey A, Wolyn David J
Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada.
Mol Nutr Food Res. 2016 Nov;60(11):2396-2412. doi: 10.1002/mnfr.201500890. Epub 2016 Aug 5.
This study investigated the effects of cooked whole asparagus (ASP) versus its equivalent level of purified flavonoid glycoside, rutin (RUT), on dextran sodium sulfate (DSS)-induced colitis and subsequent colitis recovery in mice.
C57BL/6 male mice were fed an AIN-93G basal diet (BD), or BD supplemented with 2% cooked ASP or 0.025% RUT for 2 wks prior to and during colitis induction with 2% DSS in water for 7 days, followed by 5 days colitis recovery. In colitic mice, both ASP and RUT upregulated mediators of improved barrier integrity and enhanced mucosal injury repair (e.g. Muc1, IL-22, Rho-A, Rac1, and Reg3γ), increased the proportion of mouse survival, and improved disease activity index. RUT had the greatest effect in attenuating DSS-induced colonic damage indicated by increased crypt and goblet cell restitution, reduced colonic myeloperoxidase, as well as attenuated DSS-induced microbial dysbiosis (reduced Enterobacteriaceae and Bacteroides, and increased unassigned Clostridales, Oscillospira, Lactobacillus, and Bifidobacterium).
These findings demonstrate that dietary cooked ASP and its flavonoid glycoside, RUT, may be useful in attenuating colitis severity by modulating the colonic microenvironment resulting in reduced colonic inflammation, promotion of colonic mucosal injury repair, and attenuation of colitis-associated microbial dysbiosis.
本研究调查了煮熟的全芦笋（ASP）与其同等水平的纯化黄酮糖苷芦丁（RUT）对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎及随后的结肠炎恢复的影响。
C57BL/6雄性小鼠在饮用含2% DSS的水诱导结肠炎7天之前及期间，以及随后5天的结肠炎恢复期，喂食AIN - 93G基础饮食（BD），或补充2%煮熟ASP或0.025% RUT的BD，为期2周。在结肠炎小鼠中，ASP和RUT均上调了改善屏障完整性和增强粘膜损伤修复的介质（如Muc1、IL - 22、Rho - A、Rac1和Reg3γ），增加了小鼠存活率，并改善了疾病活动指数。RUT在减轻DSS诱导的结肠损伤方面效果最佳，表现为隐窝和杯状细胞恢复增加、结肠髓过氧化物酶减少，以及减轻DSS诱导的微生物失调（肠杆菌科和拟杆菌减少，未分类的梭菌目、颤螺菌属、乳酸杆菌属和双歧杆菌属增加）。
这些发现表明，饮食中的煮熟ASP及其黄酮糖苷RUT可能通过调节结肠微环境来减轻结肠炎严重程度，从而减少结肠炎症、促进结肠粘膜损伤修复并减轻与结肠炎相关的微生物失调。
