Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada, N1G 5C9; Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada, N1G 2W1.
Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada, N1G 5C9.
J Nutr Biochem. 2018 Jun;56:215-223. doi: 10.1016/j.jnutbio.2018.02.013. Epub 2018 Feb 27.
The enriched levels of nondigestible fermentable carbohydrates and phenolic compounds found in common beans can exert immunomodulatory effects within the colon that improve gut health and mitigate the severity of colitis-associated inflammatory pathology. Prior to acute colitis onset, C57Bl/6 mice were prefed isocaloric 20% cooked navy bean (NB) or black bean (BB) diets for 3 weeks and switched to control basal diet (BD) 24 h prior to colitis induction via 5-day exposure to dextran sodium sulfate (2% w/v in drinking water)+3 days of fresh water. The severity of the acute colitis phenotype was attenuated by bean prefeeding, evidenced by reduced colon tissue inflammatory transcription factor activation (NFκB, STAT3) and inflammatory mediator levels in the colon (IL-1β, IL-6, IL-18 and MCP-1) and serum (TNFα, IL-6, IL-1β, MCP-1) versus BD (P≤.05). Additionally, biomarkers of enhanced wound repair responses were increased by bean prefeeding including colon tissue protein levels of IL-22, IL-27 and activated (i.e., GTP-bound) Cdc42 and Rac1 versus BD (P≤.05). mRNA expressions of genes involved in normal colonic epithelial function and the promotion of epithelial barrier integrity, defense and/or restitution and wound closure including MUC1, RELMβ, IgA and REG3γ were all increased in NB and BB prefed mice versus BD (P≤.05). Collectively, bean supplementation prior to colitis induction (i.e., mimicking disease relapse) primes the colonic microenvironment to attenuate the severity of the colitis inflammatory phenotype and maintain aspects of epithelial barrier function.
在普通豆类中发现的非可消化发酵碳水化合物和酚类化合物的丰富水平，可以在结肠内发挥免疫调节作用，改善肠道健康，并减轻与结肠炎相关的炎症病理的严重程度。在急性结肠炎发作之前，C57Bl/6 小鼠预先喂食等热量的 20%熟海军豆（NB）或黑豆（BB）饮食 3 周，并在结肠炎诱导前 24 小时切换到对照基础饮食（BD），通过 5 天暴露于葡聚糖硫酸钠（2%w/v 在饮用水中）+3 天淡水。豆类预先喂食减轻了急性结肠炎表型的严重程度，这表现在结肠组织炎症转录因子激活（NFκB、STAT3）和结肠中炎症介质水平（IL-1β、IL-6、IL-18 和 MCP-1）以及血清（TNFα、IL-6、IL-1β、MCP-1）降低方面与 BD（P≤.05）相比。此外，通过豆类预先喂食增加了增强伤口修复反应的生物标志物，包括结肠组织中 IL-22、IL-27 和激活（即 GTP 结合）的 Cdc42 和 Rac1 的蛋白水平与 BD（P≤.05）相比。涉及正常结肠上皮功能和促进上皮屏障完整性、防御和/或修复和伤口闭合的基因的 mRNA 表达在 NB 和 BB 预先喂食的小鼠中均高于 BD（P≤.05）。总的来说，在结肠炎诱导之前（即模拟疾病复发）补充豆类可以使结肠微环境做好准备，以减轻结肠炎炎症表型的严重程度，并维持上皮屏障功能的各个方面。
