Tokutake Takayoshi, Baba Hisami, Shimada Yuji, Takeda Wataru, Sato Keijiro, Hiroshima Yuki, Kirihara Takehiko, Shimizu Ikuo, Nakazawa Hideyuki, Kobayashi Hikaru, Ishida Fumihiro
Department of Clinical Laboratory, Nagano Red Cross Hospital, Nagano, Japan.
Department of Hematology, Nagano Red Cross Hospital, Nagano, Japan.
PLoS One. 2016 Jun 29;11(6):e0157835. doi: 10.1371/journal.pone.0157835. eCollection 2016.
The activated partial thromboplastin time (APTT) assay is a basic hemostatic assay based on the time it takes for clots to form in plasma samples after the addition of calcium chloride. It is used to screen for various coagulation disorders. Several previous reports have suggested that magnesium (Mg) might contribute to coagulation reactions by binding to specific coagulation proteins. We investigated the effects of Mg on the APTT. In healthy controls, the APTT was significantly prolonged in proportion to the increase in the concentration of magnesium chloride in the range from 2.1 to 16.7 mmol/L. Among eight samples from patients with various disorders that exhibited prolonged APTT, two samples demonstrated shorter APTT when Mg was added, both of which were from patients that were positive for lupus anticoagulant. When we examined 206 clinical APTT samples, we found that Mg shortened the APTT of two samples. These two samples were also from lupus anticoagulant-positive patients (p-value: <0.003). Our findings regarding the unique effects of exogenous Mg on the APTT of lupus anticoagulant-positive patients might shed light on the role of Mg in APTT assays and lead to the development of a novel screening method for lupus anticoagulant.
活化部分凝血活酶时间（APTT）测定是一种基本的止血测定方法，它基于在血浆样本中加入氯化钙后形成凝块所需的时间。它用于筛查各种凝血障碍。先前的几份报告表明，镁（Mg）可能通过与特定的凝血蛋白结合而参与凝血反应。我们研究了镁对APTT的影响。在健康对照中，在2.1至16.7 mmol/L范围内，随着氯化镁浓度的增加，APTT显著延长。在8份来自各种疾病且APTT延长的患者样本中，有2份样本在加入镁后APTT缩短，这两份样本均来自狼疮抗凝物阳性的患者。当我们检测206份临床APTT样本时，发现有2份样本的APTT被镁缩短。这两份样本也来自狼疮抗凝物阳性的患者（p值：<0.003）。我们关于外源性镁对狼疮抗凝物阳性患者APTT的独特影响的研究结果可能有助于阐明镁在APTT测定中的作用，并导致开发一种新的狼疮抗凝物筛查方法。
